ArticleThe Journal of biological chemistry2014
Auxin-induced rapid degradation of inhibitor of caspase-activated DNase (ICAD) induces apoptotic DNA fragmentation, caspase activation, and cell death: a cell suicide module.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Knockdown of caspase-activated DNase and B-cell lymphoma 2 inhibits cell proliferation and drug resistance in TP53-mutant multiple myeloma.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026Article
- Monoclonal antibody generation by controlled immunoglobulin gene rearrangements.Communications biology · 2025Article
- The Caspase-Activated DNase drives inflammation and contributes to defense against viral infection.Cell death and differentiation · 2024Article
- Spontaneous activity of the mitochondrial apoptosis pathway drives chromosomal defects, the appearance of micronuclei and cancer metastasis through the Caspase-Activated DNAse.Cell death & disease · 2022Article
- Dynamic study into autophagy and apoptosis during orthodontic tooth movement.Experimental and therapeutic medicine · 2021Article
- Regulated cell death in cisplatin-induced AKI: relevance ofAmerican journal of physiology. Renal physiology · 2021Article
- A non-canonical function for Centromere-associated protein-E controls centrosome integrity and orientation of cell division.Communications biology · 2021Article
- Diosgenin as a Novel Alternative Therapy for Inhibition of Growth, Invasion, and Angiogenesis Abilities of Different Glioblastoma Cell Lines.Neurochemical research · 2020Article
- An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion.Genes & development · 2019Article
- Modulating Protein Stability to Switch Toxic Protein Function On and Off in Living Cells.Plant physiology · 2019Article
- Conditional gene inactivation by combining tetracycline-mediated transcriptional repression and auxin-inducible degron-mediated degradation.Cell cycle (Georgetown, Tex.) · 2019Article
- Seh1 targets GATOR2 and Nup153 to mitotic chromosomes.Journal of cell science · 2018Article
- Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors.Nature communications · 2017Article
- Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability.Open biology · 2016Article
- Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- SCFTIR1/AFB-based auxin perception: mechanism and role in plant growth and development.The Plant cell · 2015Review
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Caspase-activated DNase (CAD) is a major apoptotic nuclease, responsible for DNA fragmentation and chromatin condensation during apoptosis. CAD is normally activated in apoptosis as a result of caspase cleavage of its inhibitory chaperone ICAD. Other aspects of CAD regulation are poorly understood. In particular, it has been unclear whether direct CAD activation in non-apoptotic living cells can trigger cell death. Taking advantage of the auxin-inducible degron (AID) system, we have developed a suicide system with which ICAD is rapidly degraded in living cells in response to the plant hormone auxin. Our studies demonstrate that rapid ICAD depletion is sufficient to activate CAD and induce cell death in DT40 and yeast cells. In the vertebrate cells, ectopic CAD activation triggered caspase activation and subsequent hallmarks of caspase-dependent apoptotic changes, including phosphatidylserine exposure and nuclear fragmentation. These observations not only suggest that CAD activation drives apoptosis through a positive feedback loop, but also identify a unique suicide system that can be used for controlling gene-modified organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.