Evidence map›Paper›PMID 25247851›Full record

ArticleNature communications2014

Rapid in vivo detection of isoniazid-sensitive Mycobacterium tuberculosis by breath test.

Seong Won Choi, Mamoudou Maiga, Mariama C Maiga, Viorel Atudorei, Zachary D Sharp, William R Bishai, Graham S Timmins

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Aptamer-Based Detection of Ampicillin in Urine Samples.Antibiotics (Basel, Switzerland) · 2020
    Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Seong Won Choi *Dept. Pharmaceutical Sciences, College of Pharmacy, University of New Mexico, Albuquerque NM 87131 USA.
Mamoudou Maiga *Center for Tuberculosis Research, Department of Medicine, Johns Hopkins University, Baltimore MD 21231 USA.
Mariama C MaigaCenter for Tuberculosis Research, Department of Medicine, Johns Hopkins University, Baltimore MD 21231 USA.
Viorel AtudoreiDept. Earth and Planetary Sciences, College of Pharmacy, University of New Mexico, Albuquerque NM 87131 USA.
Zachary D SharpDept. Earth and Planetary Sciences, College of Pharmacy, University of New Mexico, Albuquerque NM 87131 USA.
William R BishaiCenter for Tuberculosis Research, Department of Medicine, Johns Hopkins University, Baltimore MD 21231 USA.
Graham S TimminsDept. Pharmaceutical Sciences, College of Pharmacy, University of New Mexico, Albuquerque NM 87131 USA.

Funding

Mycobacterium tuberculosis Survival Regulatory GenesR01AI037856 · NIAID · JOHNS HOPKINS UNIVERSITY · PI BISHAI, WILLIAM RAMSES · 1997 to 2020
$7.2M
GENE REGULATION AND VIRULENCE IN TB AND MACR01AI036973 · NIAID · JOHNS HOPKINS UNIVERSITY · PI BISHAI, WILLIAM RAMSES · 1999 to 2014
$5.7M
Breath Test for Pseudomonas aeruginosa in CFR21AI081015 · NIAID · UNIVERSITY OF NEW MEXICO · PI TIMMINS, GRAHAM S · 2009 to 2010
$413k
SEARCH FOR GENETIC REGULATORS OF LATENCY IN M TBR29AI036973 · NIAID · JOHNS HOPKINS UNIVERSITY · PI BISHAI, WILLIAM RAMSES · 1994 to 1998
–
NIAID NIH HHS AI064386NIAID NIH HHS AI081015NIAID NIH HHS AI36973NIAID NIH HHS AI37856NIAID NIH HHS R01 AI036973NIAID NIH HHS R01 AI037856NIAID NIH HHS R21 AI081015
6 · The paper itself

Abstract

There is urgent need for rapid, point-of-care diagnostic tools for tuberculosis (TB) and drug sensitivity. Current methods based on in vitro growth take weeks, while DNA amplification can neither differentiate live from dead organisms nor determine phenotypic drug resistance. Here we show the development and evaluation of a rapid breath test for isoniazid (INH)-sensitive TB based on detection of labelled N2 gas formed specifically from labelled INH by mycobacterial KatG enzyme. In vitro data show that the assay is specific, dependent on mycobacterial abundance and discriminates between INH-sensitive and INH-resistant (S315T mutant KatG) TB. In vivo, the assay is rapid with maximal detection of (15)N2 in exhaled breath of infected rabbits within 5-10 min. No increase in (15)N2 is detected in uninfected animals, and the increases in (15)N2 are dependent on infection dose. This test may allow rapid detection of INH-sensitive TB.

Indexed as

AnimalsBacterial ProteinsBreath TestsCatalaseIsoniazidMolecular StructureMycobacterium tuberculosisNitrogen IsotopesPoint-of-Care SystemsRabbitsSensitivity and SpecificityTuberculosisBacterial ProteinsCatalaseIsoniazidkatG protein, Mycobacterium tuberculosisNitrogen Isotopes

Identifiers

PMID25247851
PMCPMC4182730

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.