ArticleJournal of innate immunity2015
Macrophage-expressed perforins mpeg1 and mpeg1.2 have an anti-bacterial function in zebrafish.
Article in Journal of innate immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 91 citations in OpenAlex.
- Perforin-2 is overexpressed in Kikuchi-Fujimoto disease.Virchows Archiv : an international journal of pathology · 2025Article
- A granulin-positive macrophage subtype in mycobacterial granulomas alleviates tissue damage by limiting excessive inflammation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Surviving on Limited Resources: Effects of Caloric Restriction on Growth, Gene Expression and Gut Microbiota in a Species With Male Pregnancy (Hippocampus erectus).Molecular ecology · 2025Article
- Review
- Accumulation and Phagocytosis of Fluorescently Visualized Macrophages Against Edwardsiella piscicida Infection in Established mpeg1.1-Transgenic Japanese Medaka Oryzias latipes.Marine biotechnology (New York, N.Y.) · 2024Article
- Dynamics of macrophage polarization supporteLife · 2024Article
- Analysis of cnidarian Gcm suggests a neuronal origin of glial EAAT1 function.Scientific reports · 2023Article
- Combined Inhibition of the TGF-β1/Smad Pathway byInternational journal of molecular sciences · 2023Article
- An arginase 2 promoter transgenic line illuminates immune cell polarisation in zebrafish.Disease models & mechanisms · 2023Article
- High-fat diet feeding triggers a regenerative response in the adult zebrafish brain.Molecular neurobiology · 2023Article
- Acute immune responses in zebrafish and evasive behavior of a parasite - who is winning?Frontiers in cellular and infection microbiology · 2023Article
- Hepatic transcriptome analyses of juvenile white bass (Frontiers in physiology · 2023Article
- Giant Triton SnailInternational journal of molecular sciences · 2022Article
- Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism.Nature communications · 2022Article
- Mpeg1 is not essential for antibacterial or antiviral immunity, but is implicated in antigen presentation.Immunology and cell biology · 2022Article
- Occurrence of foamy macrophages during the innate response of zebrafish to trypanosome infections.eLife · 2021Article
- Glucocorticoid-Induced Exacerbation of Mycobacterial Infection Is Associated With a Reduced Phagocytic Capacity of Macrophages.Frontiers in immunology · 2021Article
- Modeling Virus-Induced Inflammation in Zebrafish: A Balance Between Infection Control and Excessive Inflammation.Frontiers in immunology · 2021Review
- Breaching the Bacterial Envelope: The Pivotal Role of Perforin-2 (MPEG1) Within Phagocytes.Frontiers in immunology · 2021Review
- Illuminating Macrophage Contributions to Host-Pathogen InteractionsInfection and immunity · 2020Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Macrophage-expressed gene 1 (MPEG1) encodes an evolutionarily conserved protein with a predicted membrane attack complex/perforin domain associated with host defence against invading pathogens. In vertebrates, MPEG1/perforin-2 is an integral membrane protein of macrophages, suspected to be involved in the killing of intracellular bacteria by pore-forming activity. Zebrafish have 3 copies of MPEG1; 2 are expressed in macrophages, whereas the third could be a pseudogene. The mpeg1 and mpeg1.2 genes show differential regulation during infection of zebrafish embryos with the bacterial pathogens Mycobacterium marinum and Salmonella typhimurium. While mpeg1 is downregulated during infection with both pathogens, mpeg1.2 is infection inducible. Upregulation of mpeg1.2 is partially dependent on the presence of functional Mpeg1 and requires the Toll-like receptor adaptor molecule MyD88 and the transcription factor NFκB. Knockdown of mpeg1 alters the immune response to M. marinum infection and results in an increased bacterial burden. In Salmonella typhimurium infection, both mpeg1 and mpeg1.2 knockdown increase the bacterial burdens, but mpeg1 morphants show increased survival times. The combined results of these two in vivo infection models support the anti-bacterial function of the MPEG1/perforin-2 family and indicate that the intricate cross-regulation of the two mpeg1 copies aids the zebrafish host in combatting infection of various pathogens.
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