ArticlePloS one2014
A functional insulator screen identifies NURF and dREAM components to be required for enhancer-blocking.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 61 citations in OpenAlex.
- Identification of a cytosol-to-nucleus feedback loop that regulates neuronal microtubule nucleation.The Journal of cell biology · 2026Article
- Cooperation between architectural C2H2 proteins in CP190 recruitment to Drosophila regulatory elements.Epigenetics & chromatin · 2025Article
- 3D chromatin structures precede genome activation in Drosophila embryogenesis.Cell genomics · 2025Article
- Drosophila architectural proteins M1BP and Opbp cooperatively form the active promoter of a ribosomal protein gene.Epigenetics & chromatin · 2025Article
- New Drosophila promoter-associated architectural protein Mzfp1 interacts with CP190 and is required for housekeeping gene expression and insulator activity.Nucleic acids research · 2024Article
- SETDB1 regulates short interspersed nuclear elements and chromatin loop organization in mouse neural precursor cells.Genome biology · 2024Article
- Systematic assessment of ISWI subunits shows that NURF creates local accessibility for CTCF.Nature genetics · 2024Article
- Development of a New Model System to Study Long-Distance Interactions Supported by Architectural Proteins.International journal of molecular sciences · 2024Article
- The N-Terminal Part ofInternational journal of molecular sciences · 2023Article
- The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions inInternational journal of molecular sciences · 2023Article
- A cohesin traffic pattern genetically linked to gene regulation.Nature structural & molecular biology · 2022Article
- NURF301 contributes to gypsy chromatin insulator-mediated nuclear organization.Nucleic acids research · 2022Article
- Mechanisms of CP190 Interaction with Architectural Proteins in Drosophila Melanogaster.International journal of molecular sciences · 2021Article
- Boundaries potentiate polycomb response element-mediated silencing.BMC biology · 2021Article
- Multi-Scale Organization of theGenes · 2021Review
- Mechanism and functional role of the interaction between CP190 and the architectural protein Pita in Drosophila melanogaster.Epigenetics & chromatin · 2021Article
- Cohesin-Dependent and -Independent Mechanisms Mediate Chromosomal Contacts between Promoters and Enhancers.Cell reports · 2020Article
- The Dm-Myb Oncoprotein Contributes to Insulator Function and Stabilizes Repressive H3K27me3 PcG Domains.Cell reports · 2020Article
- Chromatin Remodelers in the 3D Nuclear Compartment.Frontiers in genetics · 2020Review
- HIPP1 stabilizes the interaction between CP190 and Su(Hw) in the Drosophila insulator complex.Scientific reports · 2019Article
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Chromatin insulators of higher eukaryotes functionally divide the genome into active and inactive domains. Furthermore, insulators regulate enhancer/promoter communication, which is evident from the Drosophila bithorax locus in which a multitude of regulatory elements control segment specific gene activity. Centrosomal protein 190 (CP190) is targeted to insulators by CTCF or other insulator DNA-binding factors. Chromatin analyses revealed that insulators are characterized by open and nucleosome depleted regions. Here, we wanted to identify chromatin modification and remodelling factors required for an enhancer blocking function. We used the well-studied Fab-8 insulator of the bithorax locus to apply a genome-wide RNAi screen for factors that contribute to the enhancer blocking function of CTCF and CP190. Among 78 genes required for optimal Fab-8 mediated enhancer blocking, all four components of the NURF complex as well as several subunits of the dREAM complex were most evident. Mass spectrometric analyses of CTCF or CP190 bound proteins as well as immune precipitation confirmed NURF and dREAM binding. Both co-localise with most CP190 binding sites in the genome and chromatin immune precipitation showed that CP190 recruits NURF and dREAM. Nucleosome occupancy and histone H3 binding analyses revealed that CP190 mediated NURF binding results in nucleosomal depletion at CP190 binding sites. Thus, we conclude that CP190 binding to CTCF or to other DNA binding insulator factors mediates recruitment of NURF and dREAM. Furthermore, the enhancer blocking function of insulators is associated with nucleosomal depletion and requires NURF and dREAM.
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