ArticleBeilstein journal of nanotechnology2014
Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii.
Article in Beilstein journal of nanotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Dynamic and Reversible Tuning of Hydrogel Viscoelasticity by Transient Polymer Interactions for Controlling Cell Adhesion.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- A Co-Polymerizable Linker for the Covalent Attachment of Fibronectin Makes pHEMA Hydrogels Cell-Adhesive.Gels (Basel, Switzerland) · 2022Article
- Human microphysiological models of airway and alveolar epithelia.American journal of physiology. Lung cellular and molecular physiology · 2021Review
- Migration of Microparticle-Containing Amoeba through Constricted Environments.ACS biomaterials science & engineering · 2020Article
- 3D Hydrogels Containing Interconnected Microchannels of Subcellular Size for Capturing Human PathogenicACS biomaterials science & engineering · 2019Article
- Ex vivo induction of regulatory T cells from conventional CD4Journal of biomedical materials research. Part A · 2018Article
- An investigation of the distribution and location of mast cells affected by the stiffness of substrates as a mechanical niche.International journal of biological sciences · 2018Article
- Mechanotransduction-modulated fibrotic microniches reveal the contribution of angiogenesis in liver fibrosis.Nature materials · 2017Article
- A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction.Scientific reports · 2017Article
- Article
- Superdiffusion dominates intracellular particle motion in the supercrowded cytoplasm of pathogenic Acanthamoeba castellanii.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMechanosensing of cells, particularly the cellular response to substrates with different elastic properties, has been discovered in recent years, but almost exclusively in mammalian cells. Much less attention has been paid to mechanosensing in other cell systems, such as in eukaryotic human pathogens.
resultsWe report here on the influence of substrate stiffness on the adhesion of the human pathogen Acanthamoebae castellanii (A. castellanii). By comparing the cell adhesion area of A. castellanii trophozoites on polydimethylsiloxane (PDMS) substrates with different Young's moduli (4 kPa, 29 kPa, and 128 kPa), we find significant differences in cell adhesion area as a function of substrate stiffness. In particular, the cell adhesion area of A. castellanii increases with a decreasing Young's modulus of the substrate.
conclusionThe dependence of A. castellanii adhesion on the elastic properties of the substrate is the first study suggesting a mechanosensory effect for a eukaryotic human pathogen. Interestingly, the main targets of A. castellanii infections in the human body are the eye and the brain, i.e., very soft environments. Thus, our study provides first hints towards the relevance of mechanical aspects for the pathogenicity of eukaryotic parasites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.