ArticleBeilstein journal of nanotechnology2014
The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles.
Article in Beilstein journal of nanotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- The Size-dependent Cytotoxicity of Amorphous Silica Nanoparticles: A Systematic Review of in vitro Studies.International journal of nanomedicine · 2020Pooled it
- Therapeutic Efficacy of Carvacrol-Loaded Mesoporous Silicate Nanoparticles Against Cryptosporidiosis.Pharmaceutics · 2026Article
- Engineering Nanoscale Frontiers: Valve Metal Oxide Nanostructures From Fundamentals to Multifunctional Biomedical Applications.Chemical record (New York, N.Y.) · 2026Review
- Tools of the trade: leveraging 3DFrontiers in pharmacology · 2026Review
- Two-Step Air/Water Oxidation Process for the Long-Lasting Photoluminescence and Biological Viability (MTT Assay) of Porous Silicon Particles.Journal of xenobiotics · 2025Article
- Impact of Milk on the Behavior and Toxicity of Nanosized SiOACS omega · 2025Article
- The Apoptosis Inhibitor Protein Survivin Is a Critical Cytoprotective Resistor against Silica-Based Nanotoxicity.Nanomaterials (Basel, Switzerland) · 2023Article
- Structural parameters of nanoparticles affecting their toxicity for biomedical applications: a review.Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology · 2023Review
- Protein corona: Friend or foe? Co-opting serum proteins for nanoparticle delivery.Advanced drug delivery reviews · 2023Review
- Gene Expression Profiling of Mono- and Co-Culture Models of the Respiratory Tract Exposed to Crystalline Quartz under Submerged and Air-Liquid Interface Conditions.International journal of molecular sciences · 2022Article
- Performance of nanoparticles for biomedical applications: TheBiophysics reviews · 2022Review
- Profiling Cisplatin Resistance in Head and Neck Cancer: A Critical Role of the VRAC Ion Channel for Chemoresistance.Cancers · 2021Article
- SWATH-MS Protocols in Human Diseases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Influence of the physicochemical features of TiORSC advances · 2020Article
- How Reversible Are the Effects of Fumed Silica on Macrophages? A Proteomics-Informed View.Nanomaterials (Basel, Switzerland) · 2020Article
- Silica Nanoparticles Provoke Cell Death Independent of p53 and BAX in Human Colon Cancer Cells.Nanomaterials (Basel, Switzerland) · 2019Article
- Amorphous silicon dioxide nanoparticles modulate immune responses in a model of allergic contact dermatitis.Scientific reports · 2019Article
- Nanosized food additives impact beneficial and pathogenic bacteria in the human gut: a simulated gastrointestinal study.NPJ science of food · 2018Article
- Re-evaluation of silicon dioxide (E 551) as a food additive.EFSA journal. European Food Safety Authority · 2018Article
- Nanoparticle core stability and surface functionalization drive the mTOR signaling pathway in hepatocellular cell lines.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Besides the lung and skin, the gastrointestinal (GI) tract is one of the main targets for accidental exposure or biomedical applications of nanoparticles (NP). Biological responses to NP, including nanotoxicology, are caused by the interaction of the NP with cellular membranes and/or cellular entry. Here, the physico-chemical characteristics of NP are widely discussed as critical determinants, albeit the exact mechanisms remain to be resolved. Moreover, proteins associate with NP in physiological fluids, forming the protein corona potentially transforming the biological identity of the particle and thus, adding an additional level of complexity for the bio-nano responses. Here, we employed amorphous silica nanoparticles (ASP) and epithelial GI tract Caco-2 cells as a model to study the biological impact of particle size as well as of the protein corona. Caco-2 or mucus-producing HT-29 cells were exposed to thoroughly characterized, negatively charged ASP of different size in the absence or presence of proteins. Comprehensive experimental approaches, such as quantifying cellular metabolic activity, microscopic observation of cell morphology, and high-throughput cell analysis revealed a dose- and time-dependent toxicity primarily upon exposure with ASP30 (Ø = 30 nm). Albeit smaller (ASP20, Ø = 20 nm) or larger particles (ASP100; Ø = 100 nm) showed a similar zeta potential, they both displayed only low toxicity. Importantly, the adverse effects triggered by ASP30/ASP30L were significantly ameliorated upon formation of the protein corona, which we found was efficiently established on all ASP studied. As a potential explanation, corona formation reduced ASP30 cellular uptake, which was however not significantly affected by ASP surface charge in our model. Collectively, our study uncovers an impact of ASP size as well as of the protein corona on cellular toxicity, which might be relevant for processes at the nano-bio interface in general.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.