ArticleBiotechnology for biofuels2014
Overexpression of the soybean transcription factor GmDof4 significantly enhances the lipid content of Chlorella ellipsoidea.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 121 citations in OpenAlex.
- Dof Transcription Factors as Integrative Regulatory Hubs Linking Carbon-Nitrogen Metabolism, Development, and Environmental Signaling in Plants.Plant, cell & environment · 2026Review
- Genome-Wide Identification of the Phosphoglycerate Kinases and Functional Analysis ofPlants (Basel, Switzerland) · 2026Article
- Enhancing soybean (Frontiers in plant science · 2026Article
- Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom.Microbial cell factories · 2025Article
- Tapping the microalgal potential: genetic precision and stress-induction for enhanced astaxanthin and biofuel production.Biotechnology for biofuels and bioproducts · 2025Review
- Engineering the Novel Extremophile AlgaACS ES&T engineering · 2025Article
- Natural hydrogen gas and engineered microalgae prevent acute lung injury in sepsis.Materials today. Bio · 2024Article
- Low-dose ionizing radiation generates a hormetic response to modify lipid metabolism in Chlorella sorokiniana.Communications biology · 2024Article
- Harnessing genetic engineering to drive economic bioproduct production in algae.Frontiers in bioengineering and biotechnology · 2024Review
- Engineering a marine microalga Chlorella sp. as the cell factory.Biotechnology for biofuels and bioproducts · 2023Article
- Isolation and Characterization of a Salt Inducible Promoter fromJournal of microbiology and biotechnology · 2023Article
- Genome-wide association study and RNA-seq identifies GmWRI1-like transcription factor related to the seed weight in soybean.Frontiers in plant science · 2023Article
- DOF transcription factors: Specific regulators of plant biological processes.Frontiers in plant science · 2023Review
- Genome-wide identification and functional analysis of Dof transcription factor family in Camelina sativa.BMC genomics · 2022Article
- Ectopic Expression ofInternational journal of molecular sciences · 2022Article
- Increased Lipids inInternational journal of molecular sciences · 2022Article
- Emerging Trends in Genetic Engineering of Microalgae for Commercial Applications.Marine drugs · 2022Review
- Comparison ofLife (Basel, Switzerland) · 2022Article
- Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture.Molecules (Basel, Switzerland) · 2021Review
- Overexpression of the Transcription FactorFrontiers in bioengineering and biotechnology · 2021Article
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe lipid content of microalgae is regarded as an important indicator for biodiesel. Many attempts have been made to increase the lipid content of microalgae through biochemical and genetic engineering. Significant lipid accumulation in microalgae has been achieved using biochemical engineering, such as nitrogen starvation, but the cell growth was severely limited. However, enrichment of lipid content in microalgae by genetic engineering is anticipated. In this study, GmDof4 from soybean (Glycine max), a transcription factor affecting the lipid content in Arabidopsis, was transferred into Chlorella ellipsoidea. We then investigated the molecular mechanism underlying the enhancement of the lipid content of transformed C. ellipsoidea.
resultsWe constructed a plant expression vector, pGmDof4, and transformed GmDof4 into C. ellipsoidea by electroporation. The resulting expression of GmDof4 significantly enhanced the lipid content by 46.4 to 52.9%, but did not affect the growth rate of the host cells under mixotrophic culture conditions. Transcriptome profiles indicated that 1,076 transcripts were differentially regulated: of these, 754 genes were significantly upregulated and 322 genes were significantly downregulated in the transgenic strains under mixotrophic culture conditions. There are 22 significantly regulated genes (|log2 ratio| >1) involved in lipid and fatty acid metabolism. Quantitative real-time PCR and an enzyme activity assay revealed that GmDof4 significantly up-regulated the gene expression and enzyme activity of acetyl-coenzyme A carboxylase, a key enzyme for fatty acid synthesis, in transgenic C. ellipsoidea cells.
conclusionsThe hetero-expression of a transcription factor GmDof4 gene from soybean can significantly increase the lipid content but not affect the growth rate of C. ellipsoidea under mixotrophic culture conditions. The increase in lipid content could be attributed to the large number of genes with regulated expression. In particular, the acetyl-coenzyme A carboxylase gene expression and enzyme activity were significantly upregulated in the transgenic cells. Our research provides a new way to increase the lipid content of microalgae by introducing a specific transcription factor to microalgae strains that can be used for the biofuel and food industries.
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