Evidence map›Paper›PMID 25246909›Full record

ArticleIranian journal of reproductive medicine2012

Effect of combined density gradient centrifugation on X- and Y- sperm separation and chromatin integrity.

Tahereh Esmaeilpour, Leila Elyasi, Soghra Bahmanpour, Alireza Ghannadi, Ahmad Monabbati, Farzaneh Dehghani, Marjaneh Kazerooni

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Iranian journal of reproductive medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Tahereh EsmaeilpourDepartment of Anatomical Sciences, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Leila ElyasiDepartment of Anatomical Sciences, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Soghra BahmanpourDepartment of Anatomical Sciences, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Alireza GhannadiIslamic Azad University, Larestan, Iran. ; Shiraz Infertility Center, Shiraz, Iran.
Ahmad MonabbatiDepartment of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.
Farzaneh DehghaniDepartment of Anatomical Sciences, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
Marjaneh KazerooniShiraz Infertility Center, Shiraz, Iran.
Shiraz University of Medical Sciences · IRIsfahan Fertility and Infertility Center · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIt has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. These methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques.

objectiveThis study compared simple density gradient methods and a combined method with albumin density gradient and PureSperm separation (alb/PureSperm) for sex preselection by double fluorescence in situ hybridization (FISH) versus chromomycin A3 staining to determine chromatin integrity. MATERIALS AND

methods30 normal semen samples were prepared with PureSperm, albumin gradients and alb/PureSperm. All samples were then stained by FISH and chromomycin A3. The results were compared with SPSS 11.5 and the Kruskal-Wallis test.

resultsThe proportion of X-bearing spermatozoa by PureSperm separation (47.58±5.67) and Y-bearing spermatozoa by albumin gradient (46.13±3.83) methods were slightly higher than in putative normal sperm samples (1:1), but there were no significant differences in the X- or Y- bearing spermatozoa counts among the three methods. Albumin gradient separation tended to underestimate abnormal spermatozoa compared to PureSperm and combined alb/PureSperm.

conclusionRoutine separation methods slightly enriched X- or Y- bearing spermatozoa, but the differences were not significant for clinical purposes. The combined alb/PureSperm method had no advantages for assessing sex ratio or chromatin integrity compared to simpler gradient methods.

Indexed as

Albumin gradientDouble-labeled fluorescence in situ hybridizationSex ratioSperm chromatinSperm separation

Identifiers

PMID25246909
PMCPMC4169681
OpenAlexW1954055081

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.