ArticleProceedings of the National Academy of Sciences of the United States of America2014
Platelet mechanosensing of substrate stiffness during clot formation mediates adhesion, spreading, and activation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
117 citing papers in PubMed, 209 citations in OpenAlex.
- Mechanotransduction of Magnetically Applied Forces Leads to Mechanical Stiffening of Platelet-Rich Plugs.Advanced healthcare materials · 2026Article
- Tissue-stiffness-driven platelet activation emerges as a potential target for breast cancer prevention and treatment.Cell communication and signaling : CCS · 2026Article
- Fibrin, from blood to bone: a review.Bone reports · 2026Review
- Platelet effector functions in inflammation.Research and practice in thrombosis and haemostasis · 2026Review
- Blood Mechanical Intelligence: From Force Sensing to Precision Mechanomedicine.Research (Washington, D.C.) · 2026Review
- Fibrin as a Versatile Fibrous Biopolymer.Sub-cellular biochemistry · 2026Review
- Investigating PolySTAT's role in clot contraction and fibrin network mechanics.Biomaterials science · 2025Article
- Super-Resolution Axial Imaging for Quantifying Piconewton Traction Forces in Live Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Microneedle-Based DNA Tension Gauge Tethers Enable In Vivo Monitoring of Cell Mechanics during Skin Tissue Regeneration.JACS Au · 2025Article
- Review
- Development of Substrate-Independent Antifouling and Bactericidal Surfaces Using Visible Light Cross-Linked Hydrogel Coatings for Biomedical Applications.Advanced healthcare materials · 2025Article
- Article
- Biomechanical platelet activation: diseases that require a new class of antiplatelet therapeutics.American journal of physiology. Cell physiology · 2025Review
- Underestimated role of macromolecular crowding in bioengineeredMaterials today. Bio · 2025Article
- Oxygen Concentration Plays a Critical Role in Fibrinogen-Mediated Platelet Activation via Inactivation of αBiomolecules · 2025Article
- Extended view on the mechanobiology of fracture healing: interplay between mechanics and inflammation.Frontiers in bioengineering and biotechnology · 2025Review
- Autoantibodies immuno-mechanically modulate platelet contractile force and bleeding risk.Nature communications · 2024Article
- Protonated-chitosan sponge with procoagulation activity for hemostasis in coagulopathy.Bioactive materials · 2024Article
- One immune cell to bind them all: platelet contribution to neurodegenerative disease.Molecular neurodegeneration · 2024Review
- Roles of Mechanosensitive Channel Piezo1 in Wound Healing and Scar Formation.Life (Basel, Switzerland) · 2024Review
57 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
As platelets aggregate and activate at the site of vascular injury to stem bleeding, they are subjected to a myriad of biochemical and biophysical signals and cues. As clot formation ensues, platelets interact with polymerizing fibrin scaffolds, exposing platelets to a large range of mechanical microenvironments. Here, we show for the first time (to our knowledge) that platelets, which are anucleate cellular fragments, sense microenvironmental mechanical properties, such as substrate stiffness, and transduce those cues into differential biological signals. Specifically, as platelets mechanosense the stiffness of the underlying fibrin/fibrinogen substrate, increasing substrate stiffness leads to increased platelet adhesion and spreading. Importantly, adhesion on stiffer substrates also leads to higher levels of platelet activation, as measured by integrin αIIbβ3 activation, α-granule secretion, and procoagulant activity. Mechanistically, we determined that Rac1 and actomyosin activity mediate substrate stiffness-dependent platelet adhesion, spreading, and activation to different degrees. This capability of platelets to mechanosense microenvironmental cues in a growing thrombus or hemostatic plug and then mechanotransduce those cues into differential levels of platelet adhesion, spreading, and activation provides biophysical insight into the underlying mechanisms of platelet aggregation and platelet activation heterogeneity during thrombus formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.