Evidence map›Paper›PMID 25245701›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

Primary cell cultures of regenerating holothurian tissues.

Samir A Bello, Ricardo J Abreu-Irizarry, José E García-Arrarás

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Advancing marine invertebrate cell line research: four key knowledge gaps.In vitro cellular & developmental biology. Animal · 2025
    Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. RNA Interference on Regenerating Holothurian Gut Tissues.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  11. Article
  12. The mesentery as the epicenter for intestinal regeneration.Seminars in cell & developmental biology · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Samir A BelloBiology Department, University of Puerto Rico, 23360, San Juan, 00931, Puerto Rico, samir040473@gmail.com.
Ricardo J Abreu-Irizarry
José E García-Arrarás

Funding

Molecular control of intestinal regenerationSC1GM084770 · NIGMS · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI GARCIA-ARRARAS, JOSE E · 2008 to 2011
$1.5M
Nervous system regeneration, molecular basis in echinodermsR15NS081686 · NINDS · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI GARCIA-ARRARAS, JOSE E · 2013 to 2013
$444k
Gene profiling of nervous system regeneration using a novel model organismR03NS065275 · NINDS · UNIVERSITY OF PUERTO RICO RIO PIEDRAS · PI GARCIA-ARRARAS, JOSE E · 2009 to 2010
$149k
NIGMS NIH HHS 1SC1GM084770-01NIGMS NIH HHS SC1 GM084770NINDS NIH HHS 1R03NS065275-01NINDS NIH HHS R03 NS065275NINDS NIH HHS R15 NS081686
6 · The paper itself

Abstract

The ability to culture different cell types is essential for answering many questions in developmental and regenerative biology. Studies in marine organisms, in particular echinoderms, have been limited by the lack of well-described cellular culture systems. Here we describe a cell culture system, for normal or regenerating holothurian cells, that allows cell characterization by immunohistochemistry and scanning electron microscopy. These cell cultures can now be used to perform multiple types of experiments in order to explore the cellular, biochemical, and genomic aspects of echinoderm regenerative properties.

Indexed as

AnimalsCells, CulturedCell ShapeDigestive SystemDisinfectionDissectionHolothuriaImmunohistochemistryPrimary Cell CultureRegeneration

Identifiers

PMID25245701
PMCPMC5426911

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.