Evidence map›Paper›PMID 25245686›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

Micro/nano-computed tomography technology for quantitative dynamic, multi-scale imaging of morphogenesis.

Chelsea L Gregg, Andrew K Recknagel, Jonathan T Butcher

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Micro-CT-Based Bone Microarchitecture Analysis of the Murine Skull.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review of quantitative multiscale imaging of breast cancer.Journal of medical imaging (Bellingham, Wash.) · 2018
    Review
  7. Article
  8. Mammal madness: is the mammal tree of life not yet resolved?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Chelsea L GreggDepartment of Biomedical Engineering, Cornell University, 304 Weill Hall, Ithaca, NY, USA.
Andrew K Recknagel
Jonathan T Butcher

Funding

Biomechanical regulation of valvulogenesisR01HL110328 · NHLBI · CORNELL UNIVERSITY · PI BUTCHER, JONATHAN TALBOT · 2011 to 2015
$1.9M
NHLBI NIH HHS R01 HL110328
6 · The paper itself

Abstract

Tissue morphogenesis and embryonic development are dynamic events challenging to quantify, especially considering the intricate events that happen simultaneously in different locations and time. Micro- and more recently nano-computed tomography (micro/nanoCT) has been used for the past 15 years to characterize large 3D fields of tortuous geometries at high spatial resolution. We and others have advanced micro/nanoCT imaging strategies for quantifying tissue- and organ-level fate changes throughout morphogenesis. Exogenous soft tissue contrast media enables visualization of vascular lumens and tissues via extravasation. Furthermore, the emergence of antigen-specific tissue contrast enables direct quantitative visualization of protein and mRNA expression. Micro-CT X-ray doses appear to be non-embryotoxic, enabling longitudinal imaging studies in live embryos. In this chapter we present established soft tissue contrast protocols for obtaining high-quality micro/nanoCT images and the image processing techniques useful for quantifying anatomical and physiological information from the data sets.

Indexed as

Imaging, Three-DimensionalMorphogenesisAnimalsAntigensContrast MediaEmbryo, MammalianMiceMicroinjectionsNanotechnologyX-Ray MicrotomographyAntigensContrast Media

Identifiers

PMID25245686
PMCPMC4460009

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.