ArticlePloS one2014
Molecular basis for antioxidant enzymes in mediating copper detoxification in the nematode Caenorhabditis elegans.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- KIN-29 SIK regulates stress-induced sleep through mitochondrial redox signaling.bioRxiv : the preprint server for biology · 2026Article
- Hormetic fasting extends Caenorhabditis elegans lifespan via H3K27 acetylation of lipid catabolism and antioxidant genes.Cell reports · 2026Article
- Chlortetracycline Disrupts Nutrient Homeostasis and Hormonal Balance in Rice Seedlings in a Dose-Dependent Manner.ACS omega · 2026Article
- Hormetic nutrient stress promotes longevity by orchestrating histone acetylation on key lipid catabolism and antioxidant defense genes.bioRxiv : the preprint server for biology · 2025Article
- Article
- Dysfunctional copper homeostasis inRedox biochemistry and chemistry · 2024Article
- Copper-mediated neurotoxicity and genetic vulnerability in the background of neurodegenerative diseases in C. elegans.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Experimental variables that impact outcomes inbioRxiv : the preprint server for biology · 2024Article
- Dysfunction in atox-1 and ceruloplasmin alters labile Cu levels and consequently Cu homeostasis inFrontiers in molecular biosciences · 2024Article
- Using Caenorhabditis elegans to assess the ecological health risks of heavy metals in soil and sediments around Dabaoshan Mine, China.Environmental science and pollution research international · 2022Article
- Metabolomics-Based Profiling ofAntioxidants (Basel, Switzerland) · 2022Article
- Molecular characterization of ethyl carbamate toxicity inToxicology reports · 2022Article
- Phenolic Compounds Reduce the Fat Content inPharmaceuticals (Basel, Switzerland) · 2020Article
- Hydrogen extends Caenorhabditis elegans longevity by reducing reactive oxygen species.PloS one · 2020Article
- Protective role of citric acid against oxidative stress induced by heavy metals in Caenorhabditis elegans.Environmental science and pollution research international · 2019Article
- The glutathione system and the related thiol network in Caenorhabditis elegans.Redox biology · 2019Review
- Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling.The Journal of cell biology · 2018Review
- Toxicity evaluation of Wanzhou watershed of Yangtze Three Gorges Reservior in the flood season in Caenorhabditis elegans.Scientific reports · 2018Article
- Metallothioneins act downstream of insulin signaling to regulate toxicity of outdoor fine particulate matter (PMToxicology research · 2016Article
- ace-3 plays an important role in phoxim resistance in Caenorhabditis elegans.Ecotoxicology (London, England) · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Antioxidant enzymes play a major role in defending against oxidative damage by copper. However, few studies have been performed to determine which antioxidant enzymes respond to and are necessary for copper detoxification. In this study, we examined both the activities and mRNA levels of SOD, CAT, and GPX under excessive copper stress in Caenorhabditis elegans, which is a powerful model for toxicity studies. Then, taking advantage of the genetics of this model, we assessed the lethal concentration (LC50) values of copper for related mutant strains. The results showed that the SOD, CAT, and GPX activities were significantly greater in treated groups than in controls. The mRNA levels of sod-3, sod-5, ctl-1, ctl-2, and almost all gpx genes were also significantly greater in treated groups than in controls. Among tested mutants, the sod-5, ctl-1, gpx-3, gpx-4, and gpx-6 variants exhibited hypersensitivity to copper. The strains with SOD or CAT over expression were reduced sensitive to copper. Mutations in daf-2 and age-1, which are involved in the insulin/insulin-like growth factor-1 signaling pathway, result in reduced sensitivity to stress. Here, we showed that LC50 values for copper in daf-2 and age-1 mutants were significantly greater than in N2 worms. However, the LC50 values in daf-16;daf-2 and daf-16;age-1 mutants were significantly reduced than in daf-2 and age-1 mutants, implying that reduced copper sensitivity is influenced by DAF-16-related functioning. SOD, CAT, and GPX activities and the mRNA levels of the associated copper responsive genes were significantly increased in daf-2 and age-1 mutants compared to N2. Additionally, the activities of SOD, CAT, and GPX were greater in these mutants than in N2 when treated with copper. Our results not only support the theory that antioxidant enzymes play an important role in copper detoxification but also identify the response and the genes involved in these processes.
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