ArticleViruses2014
A filtration based technique for simultaneous SEM and TEM sample preparation for the rapid detection of pathogens.
Article in Viruses, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Diagnostic electron microscopy in human infectious diseases - Methods and applications.Journal of microscopy · 2025Review
- The return of a forgotten ally: tabletop scanning electron microscopy in the realm of bacteriology.Frontiers in cellular and infection microbiology · 2025Review
- Nanotechnology Integration for SARS-CoV-2 Diagnosis and Treatment: An Approach to Preventing Pandemic.Nanomaterials (Basel, Switzerland) · 2021Review
- Evaluation of current diagnostic methods for COVID-19.APL bioengineering · 2020Review
- Variations in the Morphology, Mechanics and Adhesion of Persister and ResisterAntibiotics (Basel, Switzerland) · 2020Article
- Power of Scanning Electron Microscopy and Energy Dispersive X-Ray Analysis in Rapid Microbial Detection and Identification at the Single Cell Level.Scientific reports · 2020Article
- Changes in Cellular Elasticities and Conformational Properties of Bacterial Surface Biopolymers of Multidrug-ResistantCell surface (Amsterdam, Netherlands) · 2019Article
- Evaluation of antibody responses to outer membrane vesicles (OMVs) and killed whole cell ofIranian journal of microbiology · 2019Article
- Transmission Electron Microscopy Improves the Diagnostic Sensitivity in Nonbacterial Etiology of Severe Pneumonia: A Retrospective Study.The American journal of the medical sciences · 2019Article
- Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling.BMC microbiology · 2019Article
- Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field.Frontiers in microbiology · 2019Review
- Computational sensing of herpes simplex virus using a cost-effective on-chip microscope.Scientific reports · 2017Article
- The scanning electron microscope in microbiology and diagnosis of infectious disease.Scientific reports · 2016Article
- A mobile biosafety microanalysis system for infectious agents.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diagnostic electron microscopy for infectious diseases has the advantage that "everything" in the specimen can be observed, without a priori knowledge of the likely identity of the microorganisms present in the sample. The classical specimen preparation method used employs a droplet of sample, which allows particles to adsorb to a support film, and is subsequently negative stained. This "grid on drop" procedure has a sensitivity range of approximately 106 viruses per mL if no enrichment procedures are used. In the current investigation we present a novel use of filtration that allows us to detect viruses at concentrations as low as 102 viruses per mL. We present here methods based on filtration, in which total virus, and not virus concentration, is the limiting factor for detection. We show that filtration is more sensitive than conventional negative staining and can detect as few as 5 × 103 particles per sample.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.