ArticleBioMed research international2014
Quantitative analysis of pork and chicken products by droplet digital PCR.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 64 citations in OpenAlex.
- Mammalian and avian species quantification in homogenized foods: real time PCR and digital PCR as tools for label compliance controls.Scientific reports · 2024Article
- Research on quantitative detection technology of raccoon-derived ingredient adulteration in sausage products.Food science & nutrition · 2024Article
- DNA-based detection of pork content in food.Heliyon · 2023Review
- Article
- A Droplet Digital PCR Based Approach for Identification and Quantification of Porcine and Chicken Derivatives in Beef.Foods (Basel, Switzerland) · 2022Article
- Detection and Quantification of Adulterated Beef and Mutton Products by Multiplex Droplet Digital PCR.Foods (Basel, Switzerland) · 2022Article
- Quantitative monitoring of experimental and human leishmaniasis employing amastigote-specific genes.Parasitology · 2022Article
- A quantitative detection of mung bean in chestnut paste using duplex digital PCR.Current research in food science · 2022Article
- DNA Accounting: Tallying Genomes to Detect Adulterated Saffron.Foods (Basel, Switzerland) · 2021Article
- Omics-Based Analytical Approaches for Assessing Chicken Species and Breeds in Food Authentication.Molecules (Basel, Switzerland) · 2021Review
- Improving Quantitative Power in Digital PCR through Digital High-Resolution Melting.Journal of clinical microbiology · 2020Article
- A digital PCR method for identifying and quantifying adulteration of meat species in raw and processed food.PloS one · 2017Article
- Article
- Detection and quantification of beef and pork materials in meat products by duplex droplet digital PCR.PloS one · 2017Article
- Article
- Comparison of Quantitative PCR and Droplet Digital PCR Multiplex Assays for Two Genera of Bloom-Forming Cyanobacteria, Cylindrospermopsis and Microcystis.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this project, a highly precise quantitative method based on the digital polymerase chain reaction (dPCR) technique was developed to determine the weight of pork and chicken in meat products. Real-time quantitative polymerase chain reaction (qPCR) is currently used for quantitative molecular analysis of the presence of species-specific DNAs in meat products. However, it is limited in amplification efficiency and relies on standard curves based Ct values, detecting and quantifying low copy number target DNA, as in some complex mixture meat products. By using the dPCR method, we find the relationships between the raw meat weight and DNA weight and between the DNA weight and DNA copy number were both close to linear. This enabled us to establish formulae to calculate the raw meat weight based on the DNA copy number. The accuracy and applicability of this method were tested and verified using samples of pork and chicken powder mixed in known proportions. Quantitative analysis indicated that dPCR is highly precise in quantifying pork and chicken in meat products and therefore has the potential to be used in routine analysis by government regulators and quality control departments of commercial food and feed enterprises.
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Registered trials
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