ArticleBioMed research international2014
TolC promotes ExPEC biofilm formation and curli production in response to medium osmolarity.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
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- Extraintestinal traits of pathogenicity and sequence type lineages in commensalFrontiers in microbiology · 2025Article
- Efflux pumps as potential targets for biofilm inhibition.Frontiers in microbiology · 2024Review
- Effects of TolC on the pathogenicity of porcine extraintestinal pathogenicFrontiers in immunology · 2022Article
- Extraintestinal PathogenicPathogens (Basel, Switzerland) · 2021Review
- Semisynthesis and Biological Evaluation of Platencin Thioether Derivatives: Dual FabF and FabH Inhibitors against MRSA.ACS medicinal chemistry letters · 2021Article
- Biofilm Formation, Production of Matrix Compounds and Biosorption of Copper, Nickel and Lead by Different Bacterial Strains.Frontiers in microbiology · 2021Article
- Overexpression of Outer Membrane Protein X (OmpX) Compensates for the Effect of TolC Inactivation on Biofilm Formation and Curli Production in Extraintestinal PathogenicFrontiers in cellular and infection microbiology · 2018Article
- An Overview of Two-Component Signal Transduction Systems Implicated in Extra-Intestinal PathogenicFrontiers in cellular and infection microbiology · 2017Review
- Article
- Virulence characteristics of extraintestinal pathogenic Escherichia coli deletion of gene encoding the outer membrane protein X.The Journal of veterinary medical science · 2016Article
- Oxygen-Free Condition Inhibited Biofilm Formation in Extraintestinal Pathogenic Escherichia coli Strain PPECC42 Through Preventing Curli Production.Current microbiology · 2016Article
- Roles of Hcp family proteins in the pathogenesis of the porcine extraintestinal pathogenic Escherichia coli type VI secretion system.Scientific reports · 2016Article
- Article
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While a high osmolarity medium activates Cpx signaling and causes CpxR to repress csgD expression, and efflux protein TolC protein plays an important role in biofilm formation in Escherichia coli, whether TolC also responds to an osmolarity change to regulate biofilm formation in extraintestinal pathogenic E. coli (ExPEC) remains unknown. In this study, we constructed ΔtolC mutant and complement ExPEC strains to investigate the role of TolC in the retention of biofilm formation and curli production capability under different osmotic conditions. The ΔtolC mutant showed significantly decreased biofilm formation and lost the ability to produce curli fimbriae compared to its parent ExPEC strain PPECC42 when cultured in M9 medium or 1/2 M9 medium of increased osmolarity with NaCl or sucrose at 28°C. However, biofilm formation and curli production levels were restored to wild-type levels in the ΔtolC mutant in 1/2 M9 medium. We propose for the first time that TolC protein is able to form biofilm even under high osmotic stress. Our findings reveal an interplay between the role of TolC in ExPEC biofilm formation and the osmolarity of the surrounding environment, thus providing guidance for the development of a treatment for ExPEC biofilm formation.
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