ArticleBiology of sex differences2014
Sexually dimorphic characteristics of the small intestine and colon of prepubescent C57BL/6 mice.
Article in Biology of sex differences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 95 citations in OpenAlex.
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- Comparative Intestinal Transcriptomics Reveals Sex-Dependent Physiological Signatures inAnimals : an open access journal from MDPI · 2026Article
- Sexual identity of Akh neurons shapes Nucb1-dependent metabolic and reproductive plasticity.iScience · 2026Article
- Estropausal gut microbiota transplant improves measures of ovarian function in adult mice.Nature aging · 2026Article
- Sex differences in a murine model of infective endocarditis.Basic research in cardiology · 2025Article
- Serum metabolomic analysis of lumbar disc herniation at different location based on UPLC-QTOF-MS/MS: a population study.Journal of orthopaedics · 2025Article
- Exposure ofEnvironmental health perspectives · 2024Article
- Genetic hypogonadal (Gnrh1bioRxiv : the preprint server for biology · 2024Article
- XX sex chromosome complement modulates immune responses to heat-killed Streptococcus pneumoniae immunization in a microbiome-dependent manner.Biology of sex differences · 2024Article
- Sex differences in colorectal cancer: with a focus on sex hormone-gut microbiome axis.Cell communication and signaling : CCS · 2024Review
- Strain- and sex-specific differences in intestinal microhemodynamics and gut microbiota composition.Gastroenterology report · 2024Article
- XX sex chromosome complement modulates immune responses to heat-killed Streptococcus pneumoniae immunization in a microbiome-dependent manner.Research square · 2023Article
- Optical imaging of the small intestine immune compartment across scales.Communications biology · 2023Article
- Sex-Related Effects of Gut Microbiota in Metabolic Syndrome-Related Diabetic Retinopathy.Microorganisms · 2023Review
- Characterisation of the Paternal Influence on Intergenerational Offspring Cardiac and Brain Lipid Homeostasis in Mice.International journal of molecular sciences · 2023Article
- Impact of Age, Sex, and Genetic Diversity in Murine Models of the Hematopoietic Acute Radiation Syndrome (H-ARS) and the Delayed Effects of Acute Radiation Exposure (DEARE).Current stem cell reports · 2022Article
- Gut dysbiosis and age-related neurological diseases in females.Neurobiology of disease · 2022Review
- The Interplay between Androgen and Gut Microbiota: Is There a Microbiota-Gut-Testis Axis.Reproductive sciences (Thousand Oaks, Calif.) · 2022Review
- Maternal stress and the maternal microbiome have sex-specific effects on offspring development and aggressive behavior in Siberian hamsters (Phodopus sungorus).Hormones and behavior · 2022Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThere is increasing appreciation for sexually dimorphic effects, but the molecular mechanisms underlying these effects are only partially understood. In the present study, we explored transcriptomics and epigenetic differences in the small intestine and colon of prepubescent male and female mice. In addition, the microbiota composition of the colonic luminal content has been examined.
methodsAt postnatal day 14, male and female C57BL/6 mice were sacrificed and the small intestine, colon and content of luminal colon were isolated. Gene expression of both segments of the intestine was analysed by microarray analysis. DNA methylation of the promoter regions of selected sexually dimorphic genes was examined by pyrosequencing. Composition of the microbiota was explored by deep sequencing.
resultsSexually dimorphic genes were observed in both segments of the intestine of 2-week-old mouse pups, with a stronger effect in the small intestine. Amongst the total of 349 genes displaying a sexually dimorphic effect in the small intestine and/or colon, several candidates exhibited a previously established function in the intestine (i.e. Nts, Nucb2, Alox5ap and Retnlγ). In addition, differential expression of genes linked to intestinal bowel disease (i.e. Ccr3, Ccl11 and Tnfr) and colorectal cancer development (i.e. Wt1 and Mmp25) was observed between males and females. Amongst the genes displaying significant sexually dimorphic expression, nine genes were histone-modifying enzymes, suggesting that epigenetic mechanisms might be a potential underlying regulatory mechanism. However, our results reveal no significant changes in DNA methylation of analysed CpGs within the selected differentially expressed genes. With respect to the bacterial community composition in the colon, a dominant effect of litter origin was found but no significant sex effect was detected. However, a sex effect on the dominance of specific taxa was observed.
conclusionsThis study reveals molecular dissimilarities between males and females in the small intestine and colon of prepubescent mice, which might underlie differences in physiological functioning and in disease predisposition in the two sexes.
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