ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2014
A novel multiplex PCR for the simultaneous detection of Salmonella enterica and Shigella species.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 36 citations in OpenAlex.
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- Design and evaluation of a streamlined enrichment method for faster co-detection of enteric bacterial pathogens in natural soils and environmental samples.medRxiv : the preprint server for health sciences · 2026Article
- Polymerase Chain Reaction-Lateral Flow Strip for DetectingAntibiotics (Basel, Switzerland) · 2025Article
- Advances in aptamer-based biosensors for monitoring foodborne pathogens.Journal of food science and technology · 2024Review
- PathoSense: a rapid electroanalytical device platform for screening Salmonella in water samples.Mikrochimica acta · 2024Article
- Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples.Scientific reports · 2022Article
- Rapid Detection ofSensors (Basel, Switzerland) · 2021Article
- DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables.Microbial biotechnology · 2021Review
- Molecular Tools To Study Preharvest Food Safety Challenges.Microbiology spectrum · 2018Review
- Article
- A review on detection methods used for foodborne pathogens.The Indian journal of medical research · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella enterica and Shigella species are commonly associated with food and water borne infections leading to gastrointestinal diseases. The present work was undertaken to develop a sensitive and reliable PCR based detection system for simultaneous detection of Salmonella enterica and Shigella at species level. For this the conserved regions of specific genes namely ipaH1, ipaH, wbgZ, wzy and invA were targeted for detection of Shigella genus, S. flexneri, S. sonnei, S. boydii and Salmonella enterica respectively along with an internal amplification control (IAC). The results showed that twenty Salmonella and eleven Shigella spp., were accurately identified by the assay without showing non-specificity against closely related other Enterobacteriaceae organisms and also against other pathogens. Further evaluation of multiplex PCR was undertaken on 50 natural samples of chicken, eggs and poultry litter and results compared with conventional culture isolation and identification procedure. The multiplex PCR identified the presence of Salmonella and Shigella strains with a short pre-enrichment step of 5 h in peptone water and the same samples were processed by conventional procedures for comparison. Therefore, this reported multiplex PCR can serve as an alternative to the tedious time-consuming procedure of culture and identification in food safety laboratories.
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