ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2014
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Comparative Evaluation of ELISA Tests for Bovine Tuberculosis Detection: Implications for Improved Disease Control.Transboundary and emerging diseases · 2026Article
- Field Trial with Vaccine Candidates Against Bovine Tuberculosis Among Likely Infected Cattle in a Natural Transmission Setting.Vaccines · 2024Article
- First Insight into the Whole Genome Sequencing Whole Variations inMicroorganisms · 2024Article
- Limited usefulness of the ISFrontiers in veterinary science · 2024Article
- Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection ofMicrobiology spectrum · 2023Article
- Genomic analysis ofFrontiers in veterinary science · 2022Article
- Direct PCR on Tissue Samples To Detect Mycobacterium tuberculosis Complex: an Alternative to the Bacteriological Culture.Journal of clinical microbiology · 2021Article
- INCIDENCE OF RIFAMPICIN-RESISTANCE PRESUMPTIVEAfrican journal of infectious diseases · 2021Article
- ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle.The Journal of veterinary medical science · 2019Article
- Bayesian Assessment of the Accuracy of a PCR-Based Rapid Diagnostic Test for Bovine Tuberculosis in Swine.Frontiers in veterinary science · 2019Article
- Validation of a Real-Time PCR for the Detection ofFrontiers in veterinary science · 2019Article
- Multilaboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens.Journal of clinical microbiology · 2017Article
- Patterns and processes of Mycobacterium bovis evolution revealed by phylogenomic analyses.Genome biology and evolution · 2017Article
- Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil.BMC veterinary research · 2016Article
- Comparative study of Mycobacterium bovis primary isolation methods.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
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Authors and funding
15 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.
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