Evidence map›Paper›PMID 25242869›Full record

ArticleMediators of inflammation2014

Consequences of the lack of CD73 and prostatic acid phosphatase in the lymphoid organs.

Gennady G Yegutkin, Kaisa Auvinen, Marika Karikoski, Pia Rantakari, Heidi Gerke, Kati Elima, Mikael Maksimow, Ileana B Quintero, Pirkko Vihko, Marko Salmi and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mediators of inflammation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Adenosine in the Thymus.Frontiers in pharmacology · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Gennady G YegutkinMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland ; Department of Medical Microbiology and Immunology, University of Turku, 20014 Turku, Finland.
Kaisa AuvinenMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland ; National Institute for Health and Welfare, 20520 Turku, Finland.
Marika KarikoskiMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland.
Pia RantakariMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland ; Department of Physiology and Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 20014 Turku, Finland.ORCID 0000-0003-1638-5075
Heidi GerkeMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland.
Kati ElimaMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland ; Department of Medical Biochemistry and Genetics, University of Turku, 20014 Turku, Finland.
Mikael MaksimowMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland.ORCID 0000-0003-3433-700X
Ileana B QuinteroDepartment of Clinical Chemistry, and Helsinki University Hospital Laboratory, University of Helsinki, 00014 Helsinki, Finland.
Pirkko VihkoDepartment of Clinical Chemistry, and Helsinki University Hospital Laboratory, University of Helsinki, 00014 Helsinki, Finland.
Marko SalmiMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland ; Department of Medical Microbiology and Immunology, University of Turku, 20014 Turku, Finland ; National Institute for Health and Welfare, 20520 Turku, Finland.
Sirpa JalkanenMediCity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland ; Department of Medical Microbiology and Immunology, University of Turku, 20014 Turku, Finland ; National Institute for Health and Welfare, 20520 Turku, Finland.ORCID 0000-0002-1922-9732
University of Turku · FIUniversity of Helsinki · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CD73, ecto-5'-nucleotidase, is the key enzyme catalyzing the conversion of extracellular AMP to adenosine that controls vascular permeability and immunosuppression. Also prostatic acid phosphatase (PAP) possesses ecto-5'-nucleotidase/AMPase activity and is present in leukocytes. However, its role related to immune system is unknown. Therefore, we analyzed enzymatic activities and leukocyte subtypes of CD73 and PAP knockouts and generated CD73/PAP double knockout mice to elucidate the contribution of CD73 and PAP to immunological parameters. Enzymatic assays confirmed the ability of recombinant human PAP to hydrolyze [(3)H]AMP, although at much lower rate than human CD73. Nevertheless, 5'-nucleotidase/AMPase activity in splenocytes and lymphocytes from PAP(-/-) mice tended to be lower than in wild-type controls, suggesting potential contribution of PAP, along with CD73, into lymphoid AMP metabolism ex vivo. Single knockouts had decreased number of CD4(+)/CD25(+)/FoxP3 (+) regulatory T cells in thymus and CD73/PAP double knockouts exhibited reduced percentages of CD4(+) cells in spleen, regulatory T cells in lymph nodes and thymus, and CD4(+) and CD8(+) cells in blood. These findings suggest that PAP has a synergistic role together with CD73 in the immune system by contributing to the balance of leukocyte subpopulations and especially to the number of regulatory T cells in lymph nodes and thymus.

Indexed as

5'-NucleotidaseAcid PhosphataseAnimalsCD4 AntigensCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesChromatography, Thin LayerFlow CytometryForkhead Transcription FactorsHumansInterleukin-2 Receptor alpha SubunitMiceMice, KnockoutProtein Tyrosine PhosphatasesT-Lymphocytes, Regulatory5'-NucleotidaseAcid PhosphataseCD4 AntigensForkhead Transcription FactorsFoxp3 protein, mouseInterleukin-2 Receptor alpha Subunitprostatic acid phosphataseProtein Tyrosine Phosphatases

Identifiers

PMID25242869
PMCPMC4158563
OpenAlexW2044636894

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.