Evidence map›Paper›PMID 25242559›Full record

ArticleInsect biochemistry and molecular biology2014

Alkaline phosphatases and aminopeptidases are altered in a Cry11Aa resistant strain of Aedes aegypti.

Su-Bum Lee, Karlygash G Aimanova, Sarjeet S Gill

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect biochemistry and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 22 citations in OpenAlex.

  1. NativeJournal of tropical medicine · 2026
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  4. Viruses · 2022
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  5. Role of Lectin in the Response ofFrontiers in immunology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Su-Bum LeeEnvironmental Toxicology Graduate Program, University of California, Riverside, CA 92521, USA; Department of Cell Biology and Neuroscience, University of California, Riverside, CA 92521, USA.
Karlygash G AimanovaDepartment of Cell Biology and Neuroscience, University of California, Riverside, CA 92521, USA.
Sarjeet S GillEnvironmental Toxicology Graduate Program, University of California, Riverside, CA 92521, USA; Department of Cell Biology and Neuroscience, University of California, Riverside, CA 92521, USA. Electronic address: sarjeet.gill@ucr.edu.
University of California, Riverside · US

Funding

Mosquitocidal Action of Bacillus thuringiensis ToxinsR01AI066014 · NIAID · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GILL, SARJEET S · 2005 to 2020
$6.4M
NIAID NIH HHS 1R01 AI066014NIAID NIH HHS R01 AI066014
6 · The paper itself

Abstract

Bacillus thuringiensis subsp. israelensis (Bti) is widely used for the biological control of mosquito populations. However, the mechanism of Bti toxins is still not fully understood. To further elucidate the mechanism of Bti toxins, we developed an Aedes aegypti resistant strain that shows high-level resistance to Cry11Aa toxin. After 27 selections with Cry11Aa toxin, the larvae showed a 124-fold resistance ratio for Cry11Aa (strain G30). G30 larvae showed cross-resistance to Cry4Aa (66-fold resistance), less to Cry4Ba (13-fold), but not to Cry11Ba (2-fold). Midguts from these resistant larvae did not show detectable difference in the processing of the Cry11Aa toxin compared to that in susceptible larvae (WT). Brush border membrane vesicles (BBMV) from resistant larvae bound slightly less Cry11Aa compared to WT BBMV. To identify potential proteins associated with Cry11A resistance, not only transcript changes in the larval midgut were analyzed using Illumina sequencing and qPCR, but alterations of previously identified receptor proteins were investigated using immunoblots. The transcripts of 375 genes were significantly increased and those of 208 genes were down regulated in the resistant larvae midgut compared to the WT. None of the transcripts for previously identified receptors of Cry11Aa (Aedes cadherin, ALP1, APN1, and APN2) were altered in these analyses. The genes for the identified functional receptors in resistant larvae midgut did not contain any mutation in their sequences nor was there any change in their transcript expression levels compared to WT. However, ALP proteins were expressed at reduced levels (∼ 40%) in the resistant strain BBMV. APN proteins and their activity were also slightly reduced in resistance strain. The transcript levels of ALPs (AAEL013330 and AAEL015070) and APNs (AAEL008158, AAEL008162) were significantly reduced. These results strongly suggest that ALPs and APNs could be associated with Cry11Aa resistance in Ae. aegypti.

Indexed as

AedesAlkaline PhosphataseAmino Acid SequenceAminopeptidasesAnimalsBacillus thuringiensisBacterial ToxinsBase SequenceEndotoxinsGastrointestinal TractInsecticide ResistanceInsect ProteinsLarvaMicrovilliMolecular Sequence DataAlkaline PhosphataseAminopeptidasesBacterial ToxinsEndotoxinsInsect ProteinsALPAPNBacillus thuringiensisCadherinCry11Aa toxinReceptorResistant mosquitoRNA-seq

Identifiers

PMID25242559
PMCPMC4254116
OpenAlexW2094015858

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.