ArticleScientific reports2014
A sensitive method for the quantification of virion-sense and complementary-sense DNA strands of circular single-stranded DNA viruses.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Acibenzolar-Journal of pesticide science · 2026Article
- Metabolomic Analysis of Cassava Cultivars in Response to Sri Lankan Cassava Mosaic Virus Infection by Viruliferous Whiteflies.The plant pathology journal · 2025Article
- The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication.Molecular plant pathology · 2025Article
- A reporter tomato line to track replication of a geminivirus in real time and with cellular resolution.Plant biotechnology journal · 2025Article
- Transreplication Preference of the Tomato Leaf Curl Joydebpur Virus for a Noncognate Betasatellite throughMicroorganisms · 2023Article
- Construction of an Agroinfectious Clone of a Korean Isolate of Sweet Potato Symptomless Virus 1 and Comparison of Its Infectivity According to Agrobacterium tumefaciens Strains in Nicotiana benthamiana.The plant pathology journal · 2023Article
- Identification of a Novel Geminivirus inViruses · 2021Article
- Plant DNA polymerases α and δ mediate replication of geminiviruses.Nature communications · 2021Article
- Article
- A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Suppression of nbe-miR1919c-5p Expression inViruses · 2020Article
- Article
- Recent advances in understanding the replication initiator protein of the ssDNA plant viruses of the familyVirusdisease · 2019Article
- Integrated single-base resolution maps of transcriptome, sRNAome and methylome of Tomato yellow leaf curl virus (TYLCV) in tomato.Scientific reports · 2019Article
- Mimic Phosphorylation of a βC1 Protein Encoded by TYLCCNB Impairs Its Functions as a Viral Suppressor of RNA Silencing and a Symptom Determinant.Journal of virology · 2017Article
- Seed Transmission ofThe plant pathology journal · 2017Article
- Tomato yellow leaf curl virus: No evidence for replication in the insect vector Bemisia tabaci.Scientific reports · 2016Article
- Two Populations of Viral Minichromosomes Are Present in a Geminivirus-Infected Plant Showing Symptom Remission (Recovery).Journal of virology · 2016Article
- Rapid accumulation and low degradation: key parameters of Tomato yellow leaf curl virus persistence in its insect vector Bemisia tabaci.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circular single-stranded DNA (ssDNA) viruses are the smallest viruses known to infect eukaryotes. High recombination and mutation rates have conferred these viruses with an evolutionary potential that has facilitated their emergence. Their damaging effects on livestock (circoviruses) and crops (geminiviruses and nanoviruses), and the ubiquity of anelloviruses in human populations and other mammalian species, have resulted in increased interest in better understanding their epidemiology and infection mechanisms. Circular ssDNA viral replication involves the synthesis of dsDNA intermediates containing complementary-sense (CS) and virion-sense (VS) strands. Precise quantification of VS and CS accumulation during viral infections can provide insights into the molecular mechanisms underlying viral replication and the host invasion process. Although qPCR protocols for quantifying viral molecules exist, none of them discriminate VS and CS strands. Here, using a two-step qPCR protocol we have quantified VS and CS molecule accumulation during the infection process of Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Sardinia virus (TYLCSV) (genus Begomovirus, family Geminiviridae). Our results show that the VS/CS strand ratio and overall dsDNA amounts vary throughout the infection process. Moreover, we show that these values depend on the virus-host combination, and that most CS strands are present as double-stranded molecules.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.