ArticleStem cell reports2014
Tracing the origin of the HSC hierarchy reveals an SCF-dependent, IL-3-independent CD43(-) embryonic precursor.
Article in Stem cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed, 158 citations in OpenAlex.
- Generation of 3D Hemogenic Gastruloids From Mouse Embryonic Stem Cells.Bio-protocol · 2026Article
- Fetal-restricted hematopoietic progenitors arise from hemogenic endothelium in vitelline and umbilical arteries.Nature cardiovascular research · 2026Article
- Hematopoietic stem cell-independent and -dependent hematopoiesis: new insights and lineage-tracing methods.Experimental hematology · 2026Review
- CD43, but not CD41 or GPI-80, marks first definitive haematopoietic stem cells in the human embryo.Development (Cambridge, England) · 2025Article
- Article
- Unraveling hematopoietic stem cell ontogeny through single-cell multi-omics approaches.Frontiers in cell and developmental biology · 2025Review
- Bone morphogenetic protein 4 induces hematopoietic stem cell development from murine hemogenic endothelial cells in culture.Stem cell reports · 2024Article
- NR4A1 and NR4A2 orphan nuclear receptors regulate endothelial-to-hematopoietic transition in mouse hematopoietic stem cell specification.Development (Cambridge, England) · 2024Article
- Sox17 and Other SoxF-Family Proteins Play Key Roles in the Hematopoiesis of Mouse Embryos.Cells · 2024Review
- Cohesin mutations in acute myeloid leukemia.Leukemia · 2024Review
- p65 signaling dynamics drive the developmental progression of hematopoietic stem and progenitor cells through cell cycle regulation.Nature communications · 2024Article
- The molecular and cellular hematopoietic stem cell specification niche.Experimental hematology · 2024Review
- Transition of signal requirement in hematopoietic stem cell development from hemogenic endothelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.Developmental cell · 2024Article
- Autophagy regulates the maturation of hematopoietic precursors in the embryo.Nature communications · 2024Article
- Tracking early mammalian organogenesis - prediction and validation of differentiation trajectories at whole organism scale.Development (Cambridge, England) · 2024Article
- Deciphering hematopoietic stem cell development: key signaling pathways and mechanisms.Frontiers in cell and developmental biology · 2024Review
- The evolving hematopoietic niche during development.Frontiers in molecular biosciences · 2024Review
- An interactive resource of molecular signalling in the developing human haematopoietic stem cell niche.Development (Cambridge, England) · 2023Article
- Meis1 establishes the pre-hemogenic endothelial state prior to Runx1 expression.Nature communications · 2023Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Definitive hematopoietic stem cells (HSCs) develop in the aorta gonad mesonephros (AGM) region in a stepwise manner. Type I pre-HSCs express CD41 but lack CD45 expression, which is subsequently upregulated in type II pre-HSCs prior to their maturation into definitive HSCs. Here, using ex vivo modeling of HSC development, we identify precursors of definitive HSCs in the trunk of the embryonic day 9.5 (E9.5) mouse embryo. These precursors, termed here pro-HSCs, are less mature than type I and II pre-HSCs. Although pro-HSCs are CD41(+), they lack the CD43 marker, which is gradually upregulated in the developing HSC lineage. We show that stem cell factor (SCF), but not interleukin-3 (IL-3), is a major effector of HSC maturation during E9-E10. This study extends further the previously established hierarchical organization of the developing HSC lineage and presents it as a differentially regulated four-step process and identifies additional targets that could facilitate the generation of transplantable HSCs from pluripotent cells for clinical needs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.