ArticleFertility and sterility2014
Optimizing cryopreservation of human spermatogonial stem cells: comparing the effectiveness of testicular tissue and single cell suspension cryopreservation.
Article in Fertility and sterility, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Testicular tissue cryopreservation and transplantation as a strategy for feline conservation: a review of research advances.Frontiers in veterinary science · 2025Review
- Preservation of fertility in female and male prepubertal patients diagnosed with cancer.Journal of assisted reproduction and genetics · 2023Review
- Testicular Tissue Vitrification: a Promising Strategy for Male Fertility Preservation.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Immature Testicular Tissue Engineered from Weaned Mice to Adults for Prepubertal Fertility Preservation-An In Vivo Translational Study.International journal of molecular sciences · 2022Article
- Fertility Preservation and Restoration Options for Pre-Pubertal Male Cancer Patients: Current Approaches.Frontiers in endocrinology · 2022Review
- Effect of a Freezing Medium Containing Melatonin on Markers of Pre-meiotic and Post-meiotic Spermatogonial Stem Cells (SSCs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Rescue and Conservation of Male Adult Alpacas (Frontiers in veterinary science · 2021Article
- Setting Up a Cryopreservation Programme for Immature Testicular Tissue: Lessons Learned After More Than 15 Years of Experience.Clinical medicine insights. Reproductive health · 2019Article
- Wistar rats immature testicular tissue vitrification and heterotopic grafting.JBRA assisted reproduction · 2018Article
- Article
- Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation.Human reproduction update · 2016Review
- Experimental methods to preserve male fertility and treat male factor infertility.Fertility and sterility · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
objectiveTo determine whether optimal human spermatogonial stem cell (SSC) cryopreservation is best achieved with testicular tissue or single cell suspension cryopreservation. This study compares the effectiveness between these two approaches by using testicular SSEA-4+ cells, a known population containing SSCs.
designIn vitro human testicular tissues.
settingAcademic research unit. PATIENT(S): Adult testicular tissues (n=4) collected from subjects with normal spermatogenesis and normal fetal testicular tissues (n=3). INTERVENTION(S): Testicular tissue versus single cell suspension cryopreservation. MAIN OUTCOME MEASURE(S): Cell viability, total cell recovery per milligram of tissue, as well as viable and SSEA-4+ cell recovery. RESULT(S): Single cell suspension cryopreservation yielded higher recovery of SSEA-4+ cells enriched in adult SSCs, whereas fetal SSEA-4+ cell recovery was similar between testicular tissue and single cell suspension cryopreservation. CONCLUSION(S): Adult and fetal human SSEA-4+ populations exhibited differential sensitivity to cryopreservation based on whether they were cryopreserved in situ as testicular tissues or as single cells. Thus, optimal preservation of human SSCs depends on the patient's age, type of samples cryopreserved, and end points of therapeutic applications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.