ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2014
Optimized whole-genome amplification strategy for extremely AT-biased template.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Molecular Surveillance of Malaria in Nigeria Reveals a Low Frequency of Antimalarial Resistance Markers and Clonal Expansion of Chloroquine-Sensitive Infections.Open forum infectious diseases · 2026Article
- Comparative performance of microscopy, nested PCR, and real-time PCR for screening avian haemosporidian parasites in Afrotropical starlings (family Sturnidae).Parasitology research · 2023Article
- Single-cell sequencing of the small and AT-skewed genome of malaria parasites.Genome medicine · 2021Article
- First annotated draft genomes of nonmarine ostracods (Ostracoda, Crustacea) with different reproductive modes.G3 (Bethesda, Md.) · 2021Article
- Optimization of whole-genome sequencing of Plasmodium falciparum from low-density dried blood spot samples.Malaria journal · 2021Article
- Article
- Plasmodium vivax-like genome sequences shed new insights into Plasmodium vivax biology and evolution.PLoS biology · 2018Article
- Genomes of all known members of a Plasmodium subgenus reveal paths to virulent human malaria.Nature microbiology · 2018Article
- Complete avian malaria parasite genomes reveal features associated with lineage-specific evolution in birds and mammals.Genome research · 2018Article
- Direct whole-genome sequencing of Plasmodium falciparum specimens from dried erythrocyte spots.Malaria journal · 2018Article
- Aligner optimization increases accuracy and decreases compute times in multi-species sequence data.Microbial genomics · 2017Article
- Plasmodium malariae and P. ovale genomes provide insights into malaria parasite evolution.Nature · 2017Article
- Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification.Malaria journal · 2016Article
- Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Pathogen genome sequencing directly from clinical samples is quickly gaining importance in genetic and medical research studies. However, low DNA yield from blood-borne pathogens is often a limiting factor. The problem worsens in extremely base-biased genomes such as the AT-rich Plasmodium falciparum. We present a strategy for whole-genome amplification (WGA) of low-yield samples from P. falciparum prior to short-read sequencing. We have developed WGA conditions that incorporate tetramethylammonium chloride for improved amplification and coverage of AT-rich regions of the genome. We show that this method reduces amplification bias and chimera formation. Our data show that this method is suitable for as low as 10 pg input DNA, and offers the possibility of sequencing the parasite genome from small blood samples.
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