Evidence map›Paper›PMID 25240455›Full record

ArticleVirology2014

Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis.

Irina Agarkova, Brigitte Hertel, Xinzheng Zhang, Les Lane, Alexander Tchourbanov, David D Dunigan, Gerhard Thiel, Michael G Rossmann, James L Van Etten

Abstract read
In one paragraph

Article in Virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Chloroviruses.Viruses · 2019
    Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. The Autonomous Glycosylation of Large DNA Viruses.International journal of molecular sciences · 2015
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Irina AgarkovaDepartment of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583-0722, United States; Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583-0900, United States.
Brigitte HertelTechnische Universität Darmstadt, Department of Biology, Plant Membrane Biophysics, 64287 Darmstadt, Germany.
Xinzheng ZhangDepartment of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032, United States.
Les LaneDepartment of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583-0722, United States.
Alexander TchourbanovGenetics Core, University of Arizona, 246B Biological Science West, 1041 East Lowell St, Tucson, AZ 85721-0499, United States.
David D DuniganDepartment of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583-0722, United States; Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583-0900, United States.
Gerhard ThielTechnische Universität Darmstadt, Department of Biology, Plant Membrane Biophysics, 64287 Darmstadt, Germany.
Michael G RossmannDepartment of Biological Sciences, Purdue University, 240 South Martin Jischke Drive, West Lafayette, IN 47907-2032, United States.
James L Van EttenDepartment of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE 68583-0722, United States; Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583-0900, United States. Electronic address: jvanetten1@unl.edu.

Funding

STRUCTURE OF A SMALL SPHERICAL VIRUSR37AI011219 · NIAID · PURDUE UNIVERSITY WEST LAFAYETTE · PI ROSSMANN, MICHAEL G · 1986 to 2015
$6.6M
Nebraska Center for VirologyP30GM103509 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI WOOD, CHARLES · 2012 to 2014
$3.3M
STRUCTURE OF A SMALL SPHERICAL VIRUSR01AI011219 · NIAID · PURDUE UNIVERSITY WEST LAFAYETTE · PI KUHN, RICHARD J. · 1985 to 2020
$2.4M
Mentored Patient-Oriented Research Career Development AwK23RR015535 · NCRR · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI AFONJA, OLUBUMMI OLUTOYIN · 2000 to 2004
$657k
NCRR NIH HHS K23 RR015535NCRR NIH HHS P20-RR15535NIAID NIH HHS R01AI11219NIAID NIH HHS R37 AI011219NIGMS NIH HHS P30 GM103509
6 · The paper itself

Abstract

Chloroviruses infect their hosts by specifically binding to and degrading the cell wall of their algal hosts at the site of attachment, using an intrinsic digesting enzyme(s). Chlorovirus PBCV-1 stored as a lysate survived longer than virus alone, suggesting virus attachment to cellular debris may be reversible. Ghost cells (algal cells extracted with methanol) were used as a model to study reversibility of PBCV-1 attachment because ghost cells are as susceptible to attachment and wall digestion as are live cells. Reversibility of attachment to ghost cells was examined by releasing attached virions with a cell wall degrading enzyme extract. The majority of the released virions retained infectivity even after re-incubating the released virions with ghost cells two times. Thus the chloroviruses appear to have a dynamic attachment strategy that may be beneficial in indigenous environments where cell wall debris can act as a refuge until appropriate host cells are available.

Indexed as

Host-Pathogen InteractionsVirus AttachmentCalciumCell ExtractsCell WallChlorellaMicroscopy, Electron, TransmissionModels, BiologicalMucoproteinsPhycodnaviridaeVirionCalciumCell Extractslysin, gastropodaMucoproteinsCell wall degrading enzyme activityCell wall digestionChlorellaChlorovirusesPBCV-1PhycodnaviridaeReversibility of virus attachmentVirus attachmentVirus infectionVirus survival

Identifiers

PMID25240455
PMCPMC4254200

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.