ArticleMolecular cell2014
A genome-wide RNAi screen identifies opposing functions of Snai1 and Snai2 on the Nanog dependency in reprogramming.
Article in Molecular cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 59 citations in OpenAlex.
- Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice.Cell reports · 2024Article
- Snail Transcriptionally RepressesInternational journal of molecular sciences · 2024Article
- Loss of CD24 promotes radiation‑ and chemo‑resistance by inducing stemness properties associated with a hybrid E/M state in breast cancer cells.Oncology reports · 2023Article
- The Epithelial-Mesenchymal Transcription FactorCancers · 2021Article
- TINC- A Method to Dissect Regulatory Complexes at Single-Locus Resolution- Reveals an Extensive Protein Complex at the Nanog Promoter.Stem cell reports · 2020Article
- TFAP2C facilitates somatic cell reprogramming by inhibiting c-Myc-dependent apoptosis and promoting mesenchymal-to-epithelial transition.Cell death & disease · 2020Article
- Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics.Biophysical journal · 2020Article
- BMAL1 coordinates energy metabolism and differentiation of pluripotent stem cells.Life science alliance · 2020Article
- Decoding pluripotency: Genetic screens to interrogate the acquisition, maintenance, and exit of pluripotency.Wiley interdisciplinary reviews. Systems biology and medicine · 2020Review
- FOXH1 Is Regulated by NANOG and LIN28 for Early-stage Reprogramming.Scientific reports · 2019Article
- Genomic Integrity Safeguards Self-Renewal in Embryonic Stem Cells.Cell reports · 2019Article
- The role of microRNAs in embryonic stem cell and induced pluripotent stem cell differentiation in male germ cells.Journal of cellular physiology · 2019Review
- The transcription factor Slug represses p16Nature communications · 2019Article
- SNAI1 recruits HDAC1 to suppress SNAI2 transcription during epithelial to mesenchymal transition.Scientific reports · 2019Article
- Arrayed functional genetic screenings in pluripotency reprogramming and differentiation.Stem cell research & therapy · 2019Review
- Cellular trajectories and molecular mechanisms of iPSC reprogramming.Current opinion in genetics & development · 2018Review
- Snail knockdown reverses stemness and inhibits tumour growth in ovarian cancer.Scientific reports · 2018Article
- Article
- Reprogramming to pluripotency does not require transition through a primitive streak-like state.Scientific reports · 2017Article
- TGF-β Family Signaling in Embryonic and Somatic Stem-Cell Renewal and Differentiation.Cold Spring Harbor perspectives in biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
Nanog facilitates embryonic stem cell self-renewal and induced pluripotent stem cell generation during the final stage of reprogramming. From a genome-wide small interfering RNA screen using a Nanog-GFP reporter line, we discovered opposing effects of Snai1 and Snai2 depletion on Nanog promoter activity. We further discovered mutually repressive expression profiles and opposing functions of Snai1 and Snai2 during Nanog-driven reprogramming. We found that Snai1, but not Snai2, is both a transcriptional target and protein partner of Nanog in reprogramming. Ectopic expression of Snai1 or depletion of Snai2 greatly facilitates Nanog-driven reprogramming. Snai1 (but not Snai2) and Nanog cobind to and transcriptionally activate pluripotency-associated genes including Lin28 and miR-290-295. Ectopic expression of miR-290-295 cluster genes partially rescues reprogramming inefficiency caused by Snai1 depletion. Our study thus uncovers the interplay between Nanog and mesenchymal factors Snai1 and Snai2 in the transcriptional regulation of pluripotency-associated genes and miRNAs during the Nanog-driven reprogramming process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.