Evidence map›Paper›PMID 25239909›Full record

ArticleApplied and environmental microbiology2014

Spatially Organized Films from Bdellovibrio bacteriovorus Prey Lysates.

Megan A Ferguson, Megan E Núñez, Hyeong-Jin Kim, Shana Goffredi, Elya Shamskhou, Leanna Faudree, Evan Chang, Rebecca M Landry, Andrew Ma, Da-Eun Choi and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Biotechnological Potential ofFrontiers in microbiology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Megan A FergusonDepartment of Chemistry, State University of New York at New Paltz, New Paltz, New York, USA.
Megan E NúñezDepartment of Chemistry, Wellesley College, Wellesley, Massachusetts, USA.
Hyeong-Jin KimDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Shana GoffrediDepartment of Biology, Occidental College, Los Angeles, California, USA.
Elya ShamskhouDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Leanna FaudreeDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Evan ChangDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Rebecca M LandryDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Andrew MaDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Da-Eun ChoiDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Nicholas ThomasDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Jaclyn SchmittDepartment of Chemistry, Occidental College, Los Angeles, California, USA.
Eileen M SpainDepartment of Chemistry, Occidental College, Los Angeles, California, USA emspain@oxy.edu.
Occidental College · USSUNY New Paltz · USWellesley College · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bdellovibrio bacteriovorus is a Gram-negative predator of other Gram-negative bacteria. Interestingly, Bdellovibrio bacteriovorus 109J cells grown in coculture with Escherichia coli ML-35 prey develop into a spatially organized two-dimensional film when located on a nutrient-rich surface. From deposition of 10 μl of a routine cleared coculture of B. bacteriovorus and E. coli cells, the cells multiply into a macroscopic community and segregate into an inner, yellow circular region and an outer, off-white region. Fluorescence in situ hybridization and atomic force microscopy measurements confirm that the mature film is spatially organized into two morphologically distinct Bdellovibrio populations, with primarily small, vibroid cells in the center and a complex mixture of pleomorphic cells in the outer radii. The interior region cell population exhibits the hunting phenotype while the outer region cell subpopulation does not. Crowding and high nutrient availability with limited prey appear to favor diversification of the B. bacteriovorus population into two distinct, thriving subpopulations and may be beneficial to the persistence of B. bacteriovorus in biofilms.

Indexed as

Microbial InteractionsBdellovibrioCulture MediaEscherichia coliIn Situ Hybridization, FluorescenceMicroscopy, Atomic ForceCulture Media

Identifiers

PMID25239909
PMCPMC4249199
OpenAlexW2052535860

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.