ArticleApplied and environmental microbiology2014
Fine-Tuning of the Fatty Acid Pathway by Synthetic Antisense RNA for Enhanced (2S)-Naringenin Production from l-Tyrosine in Escherichia coli.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 78 citations in OpenAlex.
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- ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism.Nature communications · 2024Article
- Enhanced biosynthesis of poly(3-hydroxybutyrate) in engineered strains of Pseudomonas putida via increased malonyl-CoA availability.Microbial biotechnology · 2024Article
- Systems biology of industrial oxytetracycline production in Streptomyces rimosus: the secrets of a mutagenized hyperproducer.Microbial cell factories · 2023Article
- Synthetic Biology-Driven Microbial Production of Resveratrol: Advances and Perspectives.Frontiers in bioengineering and biotechnology · 2022Review
- Resveratrol Production in Yeast Hosts: Current Status and Perspectives.Biomolecules · 2021Review
- Development of antisense RNA-mediated quantifiable inhibition for metabolic regulation.Metabolic engineering communications · 2021Article
- Downregulating of hemB via synthetic antisense RNAs for improving 5-aminolevulinic acid production in3 Biotech · 2021Article
- Fermentation and Metabolic Pathway Optimization to De Novo Synthesize (2S)-Naringenin inJournal of microbiology and biotechnology · 2020Article
- Coupling metabolic addiction with negative autoregulation to improve strain stability and pathway yield.Metabolic engineering · 2020Article
- Engineering intracellular malonyl-CoA availability in microbial hosts and its impact on polyketide and fatty acid synthesis.Applied microbiology and biotechnology · 2020Review
- Toward fine-tuned metabolic networks in industrial microorganisms.Synthetic and systems biotechnology · 2020Article
- Metabolic Engineering of Microorganisms for the Production of Flavonoids.Frontiers in bioengineering and biotechnology · 2020Review
- Development of an autonomous and bifunctional quorum-sensing circuit for metabolic flux control in engineeredProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Advances in engineered trans-acting regulatory RNAs and their application in bacterial genome engineering.Journal of industrial microbiology & biotechnology · 2019Review
- Modulating heterologous pathways and optimizing fermentation conditions for biosynthesis of kaempferol and astragalin from naringenin in Escherichia coli.Journal of industrial microbiology & biotechnology · 2019Article
- Regulation of ATP levels in Escherichia coli using CRISPR interference for enhanced pinocembrin production.Microbial cell factories · 2018Article
- Engineering Escherichia coli for Glutarate Production as the CApplied and environmental microbiology · 2018Article
- Sensor-regulator and RNAi based bifunctional dynamic control network for engineered microbial synthesis.Nature communications · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Malonyl coenzyme A (malonyl-CoA) is an important precursor for the synthesis of natural products, such as polyketides and flavonoids. The majority of this cofactor often is consumed for producing fatty acids and phospholipids, leaving only a small amount of cellular malonyl-CoA available for producing the target compound. The tuning of malonyl-CoA into heterologous pathways yields significant phenotypic effects, such as growth retardation and even cell death. In this study, fine-tuning of the fatty acid pathway in Escherichia coli with antisense RNA (asRNA) to balance the demands on malonyl-CoA for target-product synthesis and cell health was proposed. To establish an efficient asRNA system, the relationship between sequence and function for asRNA was explored. It was demonstrated that the gene-silencing effect of asRNA could be tuned by directing asRNA to different positions in the 5'-UTR (untranslated region) of the target gene. Based on this principle, the activity of asRNA was quantitatively tailored to balance the need for malonyl-CoA in cell growth and the production of the main flavonoid precursor, (2S)-naringenin. Appropriate inhibitory efficiency of the anti-fabB/fabF asRNA improved the production titer by 431% (391 mg/liter). Therefore, the strategy presented in this study provided a useful tool for the fine-tuning of endogenous gene expression in bacteria.
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