ArticlePlant physiology2014
Association of the P6 protein of Cauliflower mosaic virus with plasmodesmata and plasmodesmal proteins.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.
- A comparative meta-proteomic pipeline for the identification of plasmodesmata proteins and regulatory conditions in diverse plant species.BMC biology · 2022Pooled it
- Role of Plasmodesmata During Plant-Pathogen Interactions.The plant pathology journal · 2026Review
- Silencing of Putative Plasmodesmata-Associated GenesPlants (Basel, Switzerland) · 2025Article
- Endogenous Caulimovirids: Fossils, Zombies, and Living in Plant Genomes.Biomolecules · 2023Review
- Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus.AMB Express · 2022Article
- Plasmodesmata Structural Components and Their Role in Signaling and Plant Development.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Review
- Genome Editing for Plasmodesmal Biology.Frontiers in plant science · 2021Review
- Creating Contacts Between Replication and Movement at Plasmodesmata - A Role for Membrane Contact Sites in Plant Virus Infections?Frontiers in plant science · 2020Review
- Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells.BioMed research international · 2020Article
- Small hydrophobic viral proteins involved in intercellular movement of diverse plant virus genomes.AIMS microbiology · 2020Review
- Cauliflower mosaic virus transactivator protein (TAV) can suppress nonsense-mediated decay by targeting VARICOSE, a scaffold protein of the decapping complex.Scientific reports · 2019Article
- Plant defense against virus diseases; growth hormones in highlights.Plant signaling & behavior · 2019Review
- Split green fluorescent protein as a tool to study infection with a plant pathogen, Cauliflower mosaic virus.PloS one · 2019Article
- An aromatic amino acid and associated helix in the C-terminus of the potato leafroll virus minor capsid protein regulate systemic infection and symptom expression.PLoS pathogens · 2018Article
- Inducible transgenic tobacco system to study the mechanisms underlying chlorosis mediated by the silencing of chloroplast heat shock protein 90.Virusdisease · 2017Article
- Article
- Setting Up Shop: The Formation and Function of the Viral Factories ofFrontiers in plant science · 2017Review
- Chloroplast in Plant-Virus Interaction.Frontiers in microbiology · 2016Review
- Mutations within A 35 amino acid region of P6 influence self-association, inclusion body formation, and Caulimovirus infectivity.Virology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The P6 protein of Cauliflower mosaic virus (CaMV) is responsible for the formation of inclusion bodies (IBs), which are the sites for viral gene expression, replication, and virion assembly. Moreover, recent evidence indicates that ectopically expressed P6 inclusion-like bodies (I-LBs) move in association with actin microfilaments. Because CaMV virions accumulate preferentially in P6 IBs, we hypothesized that P6 IBs have a role in delivering CaMV virions to the plasmodesmata. We have determined that the P6 protein interacts with a C2 calcium-dependent membrane-targeting protein (designated Arabidopsis [Arabidopsis thaliana] Soybean Response to Cold [AtSRC2.2]) in a yeast (Saccharomyces cerevisiae) two-hybrid screen and have confirmed this interaction through coimmunoprecipitation and colocalization assays in the CaMV host Nicotiana benthamiana. An AtSRC2.2 protein fused to red fluorescent protein (RFP) was localized to the plasma membrane and specifically associated with plasmodesmata. The AtSRC2.2-RFP fusion also colocalized with two proteins previously shown to associate with plasmodesmata: the host protein Plasmodesmata-Localized Protein1 (PDLP1) and the CaMV movement protein (MP). Because P6 I-LBs colocalized with AtSRC2.2 and the P6 protein had previously been shown to interact with CaMV MP, we investigated whether P6 I-LBs might also be associated with plasmodesmata. We examined the colocalization of P6-RFP I-LBs with PDLP1-green fluorescent protein (GFP) and aniline blue (a stain for callose normally observed at plasmodesmata) and found that P6-RFP I-LBs were associated with each of these markers. Furthermore, P6-RFP coimmunoprecipitated with PDLP1-GFP. Our evidence that a portion of P6-GFP I-LBs associate with AtSRC2.2 and PDLP1 at plasmodesmata supports a model in which P6 IBs function to transfer CaMV virions directly to MP at the plasmodesmata.
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