ArticleBiology open2014
Myosin II does not contribute to wound repair in Dictyostelium cells.
Article in Biology open, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Actomyosin dynamics in detached cells: linking clutch model to cell migration and cytokinesis.BMC molecular and cell biology · 2025Article
- Please inhibit responsibly: Natural and synthetic actin toxins as useful tools in cell biology.Molecular biology of the cell · 2025Review
- Wound Repair of the Cell Membrane: Lessons fromCells · 2024Review
- Field model for multistate lateral diffusion of various transmembrane proteins observed in living Dictyostelium cells.Journal of cell science · 2023Article
- Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.Cells · 2022Article
- Review
- Article
- Autocrine insulin pathway signaling regulates actin dynamics in cell wound repair.PLoS genetics · 2020Article
- CaCells · 2020Review
- Into the breach: how cells cope with wounds.Open biology · 2018Review
- A study of wound repair in Dictyostelium cells by using novel laserporation.Scientific reports · 2018Article
- A novel low-power laser-mediated transfer of foreign molecules into cells.Scientific reports · 2016Article
- Cell healing: Calcium, repair and regeneration.Seminars in cell & developmental biology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells are always subjected to mechanical stresses, resulting in wounds of the cell membrane, but cells are able to repair and reseal their wounded membrane. Previous reports have shown that actin and myosin II accumulate around the wound and that the constriction of this purse-string closes the membrane pore. Here, we developed a microsurgical wound assay to assess wound repair in Dictyostelium cells. Fluorescent dye that had been incorporated into the cells leaked out for only 2-3 sec after wounding, and a GFP-derived, fluorescent Ca(2+) sensor showed that intracellular Ca(2+) transiently increased immediately after wounding. In the absence of external Ca(2+), the cell failed to repair itself. During the repair process, actin accumulated at the wounded sites but myosin II did not. The wounds were repaired even in myosin II null cells to a comparable degree as the wild-type cells, suggesting that myosin II does not contribute to wound repair. Thus, the actomyosin purse-string constriction model is not a common mechanism for wound repair in eukaryotic cells, and this discrepancy may arise from the difference in cell size.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.