ArticleMolecular endocrinology (Baltimore, Md.)2014
Targeted gene disruption in zebrafish reveals noncanonical functions of LH signaling in reproduction.
Article in Molecular endocrinology (Baltimore, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 113 citations in OpenAlex.
- Review
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- Emerging Perspectives on Gonadotropin Regulation in Vertebrates Revealed by the Discovery of FSH-RH in Teleosts.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Effect of genomic regions harboring putative lethal haplotypes on reproductive performance in closed experimental selection lines of Nellore cattle.Scientific reports · 2025Article
- The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretion.eLife · 2024Article
- Combined Pituitary Hormone Deficiency inInternational journal of molecular sciences · 2024Article
- New insights into the all-testis differentiation in zebrafish with compromised endogenous androgen and estrogen synthesis.PLoS genetics · 2024Article
- Gonacin: A germ cell-derived hormone with glucogenic, orexigenic, and gonadal activities.iScience · 2023Article
- Chemogenetic Depletion of Hypophysiotropic GnRH Neurons Does Not Affect Fertility in Mature Female Zebrafish.International journal of molecular sciences · 2022Article
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- Transcriptomes of testis and pituitary from male Nile tilapia (O. niloticus L.) in the context of social status.PloS one · 2022Article
- The Ovarian Transcriptome at the Early Stage of Testis Removal-Induced Male-To-Female Sex Change in the Protandrous Black PorgyFrontiers in genetics · 2022Article
- Sex Determination and Differentiation in Teleost: Roles of Genetics, Environment, and Brain.Biology · 2021Review
- Gnrh2 maintains reproduction in fasting zebrafish through dynamic neuronal projection changes and regulation of gonadotropin synthesis, oogenesis, and reproductive behaviors.Scientific reports · 2021Article
- Improving the sexual activity and reproduction of female zebrafish with high testosterone levels.Scientific reports · 2021Article
- Unconventional Actions of Glycoprotein Hormone Subunits: A Comprehensive Review.Frontiers in endocrinology · 2021Review
- Targeted mutation of secretogranin-2 disrupts sexual behavior and reproduction in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Synergistic Combination of Exogenous Hormones to Improve the Spawning and Post-spawning Survival of Female Yellow Catfish.Frontiers in genetics · 2020Article
- AnFrontiers in endocrinology · 2020Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pivotal role of gonadotropin signaling in regulating gonadal development and functions has attracted much research attention in the past 2 decades. However, the precise physiological role of gonadotropin signaling is still largely unknown in fish. In this study, we have established both LH β-subunit (lhb) and LH receptor (lhr) knockout zebrafish lines by transcription activator-like effector nucleases. Intriguingly, both homozygous lhb and lhr mutant male fish are fertile. The fertilization rate, sperm motility, and histological structure of the testis were not affected in either lhb or lhr mutant males. On the contrary, homozygous lhb mutant females are infertile, whereas homozygous lhr mutant females are fertile. Folliculogenesis was not affected in either lhb or lhr mutants, but oocyte maturation and ovulation were disrupted in lhb mutant, whereas only ovulation was affected in lhr mutant. Differential expression of genes in the ovary involved in steroidogenesis, oocyte maturation, and ovulation was found between the lhb and lhr mutants. These data demonstrate the essential role of LH signaling in oocyte maturation and ovulation, and support the notion that LH acts through the FSH receptor in the absence of LH receptor. Moreover, the defects of lhb mutant could be partially restored by administration of human chorionic gonadotropin. This in vivo evidence in the present study demonstrates, for the first time in any vertebrate species, that LH signaling is indispensable in female reproduction but not in male reproduction. LH signaling is demonstrated to control oocyte maturation and ovulation in the ovary.
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