ArticlePloS one2014
Multi-level kinetic model of mRNA delivery via transfection of lipoplexes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Multiscale modeling guided potency assessment of mRNA-lipid nanoparticles.Molecular therapy. Nucleic acids · 2026Article
- SIMPROV: Provenance capturing for simulation studies.PloS one · 2025Article
- Realizing time-staggered expression of nucleic acid-encoded proteins by co-delivery of messenger RNA and plasmid DNA on a single nanocarrier.Drug delivery and translational research · 2024Article
- Modeling onActa pharmaceutica Sinica. B · 2024Article
- Article
- Matrix stiffness regulates lipid nanoparticle-mRNA delivery in cell-laden hydrogels.Nanomedicine : nanotechnology, biology, and medicine · 2022Article
- Identifiability analysis for models of the translation kinetics after mRNA transfection.Journal of mathematical biology · 2022Article
- Intravesical delivery ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Gene-circuit therapy on the horizon: synthetic biology tools for engineered therapeutics.Acta biochimica Polonica · 2021Review
- Protein Expression Correlates Linearly with mRNA Dose over Up to Five Orders of Magnitude In Vitro and In Vivo.Biomedicines · 2021Article
- Designing biomaterials for the delivery of RNA therapeutics to stimulate bone healing.Materials today. Bio · 2021Review
- Strategies for simultaneous and successive delivery of RNA.Journal of molecular medicine (Berlin, Germany) · 2020Article
- Multi-experiment nonlinear mixed effect modeling of single-cell translation kinetics after transfection.NPJ systems biology and applications · 2019Article
- Biomedical applications of mRNA nanomedicine.Nano research · 2018Article
- Quantitative Systems Pharmacology Model of hUGT1A1-modRNA Encoding for the UGT1A1 Enzyme to Treat Crigler-Najjar Syndrome Type 1.CPT: pharmacometrics & systems pharmacology · 2018Article
- Delivery of modified mRNA encoding vesicular stomatitis virus matrix protein for colon cancer gene therapy.RSC advances · 2018Article
- Tracking the Evolution of Transiently Transfected Individual Cells in a Microfluidic Platform.Scientific reports · 2018Article
- Characterizing exogenous mRNA delivery, trafficking, cytoplasmic release and RNA-protein correlations at the level of single cells.Nucleic acids research · 2017Article
- Biomaterials for mRNA delivery.Biomaterials science · 2015Article
- Modeling for (physical) biologists: an introduction to the rule-based approach.Physical biology · 2015Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent work on the use of mRNA lipoplexes for gene delivery demonstrates the need for a mathematical model that simulates and predicts kinetics and transfection efficiency. The small copy numbers involved make it necessary to use stochastic models and include statistical analysis of the variation observed in the experimental data. The modeling requirements are further complicated by the multi-level nature of the problem, where mRNA molecules are contained in lipoplexes, which are in turn contained in endosomes, where each of these entities displays a behavior of its own. We have created a mathematical model that reproduces both the time courses and the statistical variance observed in recent experiments using single-cell tracking of GFP expression after transfection. By applying a few key simplifications and assumptions, we have limited the number of free parameters to five, which we optimize to match five experimental determinants by means of a simulated annealing algorithm. The models demonstrate the need for modeling of nested species in order to reproduce the shape of the dose-response and expression-level curves.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.