Evidence map›Paper›PMID 25237826›Full record

ReviewNature reviews. Molecular cell biology2014

Three-dimensional organotypic culture: experimental models of mammalian biology and disease.

Eliah R Shamir, Andrew J Ewald

Registry-linked trialAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Nature reviews. Molecular cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05352165 (A Prospective Multicenter Randomized Controlled Trial of the Clinical Efficacy of Neoadjuvant Therapy Based on Organoids Drug Sensitivity Versus Empirical Neoadjuvant Therapy in the Treatment of Advanced Rectal Cancer), which is not on this map. Cited by 369 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
369citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05352165 naunknown statusnot on this mapstarted 2023, after this paper: background citation

A Prospective Multicenter Randomized Controlled Trial of the Clinical Efficacy of Neoadjuvant Therapy Based on Organoids Drug Sensitivity Versus Empirical Neoadjuvant Therapy in the Treatment of Advanced Rectal Cancer

TypeinterventionalSponsorShanghai Minimally Invasive Surgery CenterRan2023 to 2025Enrolled192ConditionsNeoadjuvant TherapyArmsstandard long-term therapy, FOLFOX and standard long-term radiotherapy, FOLFIRI and standard long-term radiotherapy, 5-FU and standard long-term radiotherapy, 5-FU and pembrolizumab and standard long-term radiotherapy
3 · Its place in the literature

Who cites it

369 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Three dimensional (3D) gingival models in periodontal research: a systematic review.Journal of materials science. Materials in medicine · 2023
    Pooled it
  2. Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020
    Pooled it
  3. Article
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  7. Review
  8. Review
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  12. Review
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  14. An Intelligent Magneto-Mechanical Platform for Cellular Sensing in 3D Microenvironments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Review
  20. Article

309 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Eliah R ShamirDepartments of Cell Biology and Oncology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University, 855 North Wolfe Street, Baltimore, Maryland 21205, USA.
Andrew J EwaldDepartments of Cell Biology and Oncology, Center for Cell Dynamics, School of Medicine, Johns Hopkins University, 855 North Wolfe Street, Baltimore, Maryland 21205, USA.

Funding

Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI Rosalie Wollett · 1985 to 2026
$208.6M
MEDICAL SCIENTIST TRAINING PROGRAMT32GM007309 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI COX, ANDREA L · 1985 to 2019
$43.2M
Analysis of the Signaling and Mechanical Cues Promoting Invasion in MelanomaU01CA155758 · NCI · YALE UNIVERSITY · PI LAUFFENBURGER, DOUGLAS A, LEVCHENKO, ANDRE · 2011 to 2015
$1.8M
NCI NIH HHS P30 CA006973NCI NIH HHS U01 CA155758NIGMS NIH HHS T32 GM007309
6 · The paper itself

Abstract

Mammalian organs are challenging to study as they are fairly inaccessible to experimental manipulation and optical observation. Recent advances in three-dimensional (3D) culture techniques, coupled with the ability to independently manipulate genetic and microenvironmental factors, have enabled the real-time study of mammalian tissues. These systems have been used to visualize the cellular basis of epithelial morphogenesis, to test the roles of specific genes in regulating cell behaviours within epithelial tissues and to elucidate the contribution of microenvironmental factors to normal and disease processes. Collectively, these novel models can be used to answer fundamental biological questions and generate replacement human tissues, and they enable testing of novel therapeutic approaches, often using patient-derived cells.

Indexed as

Cell Culture TechniquesAnimalsCell ProliferationCellular MicroenvironmentEpithelial CellsEpitheliumGene Expression RegulationMammalsMorphogenesisOrgan Culture Techniques

Identifiers

PMID25237826
PMCPMC4352326

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.