ArticleFrontiers in plant science2014
WHIRLY1 is a major organizer of chloroplast nucleoids.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- The GT1 domain of RNase J ensures RNA quality control through dsRNA binding in Arabidopsis plastids.Nucleic acids research · 2026Article
- Article
- PEN1 catalyses RNA primer removal during plastid DNA replication in maize.Nature plants · 2025Article
- Article
- Genome-Wide Identification and Characterization of the mTERF Gene Family in Spinach and the Role ofPlants (Basel, Switzerland) · 2025Article
- Over-accumulation of chloroplast-nucleus located WHIRLY1 in barley leads to a decrease in growth and an enhanced stress resistance.The Plant journal : for cell and molecular biology · 2024Article
- Characterization of the DNA accessibility of chloroplast genomes in grasses.Communications biology · 2024Article
- How do barley plants with impaired photosynthetic light acclimation survive under high-light stress?Planta · 2023Article
- WHIRLY proteins maintain seed longevity by effects on seed oxygen signalling during imbibition.The Biochemical journal · 2023Article
- WHIRLY protein functions in plants.Food and energy security · 2023Review
- Redox Signaling in Plant Heat Stress Response.Antioxidants (Basel, Switzerland) · 2023Review
- OsWHY1 Interacts with OsTRX z and is Essential for Early Chloroplast Development in Rice.Rice (New York, N.Y.) · 2022Article
- WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles.The Biochemical journal · 2022Article
- The plastid-nucleus localized DNA-binding protein WHIRLY1 is required for acclimation of barley leaves to high light.Planta · 2022Article
- WHIRLIES Are Multifunctional DNA-Binding Proteins With Impact on Plant Development and Stress Resistance.Frontiers in plant science · 2022Review
- Article
- Genome communication in plants mediated by organelle-n-ucleus-located proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Retrograde signals from endosymbiotic organelles: a common control principle in eukaryotic cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- WHIRLY2 plays a key role in mitochondria morphology, dynamics, and functionality inPlant direct · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
WHIRLY1 is an abundant protein of chloroplast nucleoids, which has also been named pTAC-1 with regard to its detection in the proteome of transcriptionally active chromosomes (TAC). In barley primary foliage leaves, expression of the WHIRLY1 gene is highest at the base whereas protein accumulation is highest in the middle of the leaf where young developing chloroplasts are found. In order to elucidate the function of WHIRLY1 in chloroplast nucleoids, transgenic barley plants with an RNAi-mediated knock-down of the HvWHIRLY1 gene (RNAi-W1) were generated. The homozygous RNAi-W1-7 plants, barely containing traces of the WHIRLY1 protein, were chosen for detailed analyses of nucleoids. Nucleic acid specific-staining with YO-PRO®-1 revealed that in comparison to wild type chloroplasts, which have multiple small nucleoids attached to thylakoids, chloroplasts of the transgenic plants contain large irregularly formed patches of DNA besides nucleoids that are similar in size and shape to those of wild type chloroplasts. In large electron lucent areas, filamentous structures were detected by conventional transmission electron microscopy. Analyses of ptDNA levels by both DNA dot-blot hybridization and quantitative PCR showed that leaves of the transgenic plants have a two- to three-fold higher level of ptDNA than the wild type. The higher ptDNA level in RNAi-W1 plants coincided with an enhanced expression of the gene encoding a putative organelle targeted DNA polymerase in the mid part of primary foliage leaves. Furthermore, overexpression of the barley WHIRLY1 gene in E. coli cells revealed a higher compaction of bacterial nucleoids. These results suggest that WHIRLY1 belongs to the group of plastid nucleoid associated proteins (ptNAP) having a function in compacting a subpopulation of chloroplast nucleoids thereby affecting DNA replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.