ArticleApplied microbiology and biotechnology2014
Transcriptomic analysis reveals new regulatory roles of Clp signaling in secondary metabolite biosynthesis and surface motility in Lysobacter enzymogenes OH11.
Article in Applied microbiology and biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 70 citations in OpenAlex.
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- Loss of Flagella-Related Genes Enables a Nonflagellated, Fungal-Predating Bacterium To Strengthen the Synthesis of an Antifungal Weapon.Microbiology spectrum · 2023Article
- Diguanylate Cyclase and Phosphodiesterase Interact To Maintain the Specificity of Cyclic di-GMP Signaling in the Regulation of Antibiotic Synthesis in Lysobacter enzymogenes.Applied and environmental microbiology · 2022Article
- Comparative genomics provides insights into the potential biocontrol mechanism of twoFrontiers in microbiology · 2022Article
- Outer Membrane Vesicle-Mediated Codelivery of the Antifungal HSAF Metabolites and Lytic Polysaccharide Monooxygenase in the PredatoryACS chemical biology · 2021Article
- Coordinated control of the type IV pili and c-di-GMP-dependent antifungal antibiotic production in Lysobacter by the response regulator PilR.Molecular plant pathology · 2021Article
- An Antifungal Polycyclic Tetramate Macrolactam, Heat-Stable Antifungal Factor (HSAF), Is a Novel Oxidative Stress Modulator in Lysobacter enzymogenes.Applied and environmental microbiology · 2021Article
- Separation of Heat-Stable Antifungal Factor FromFrontiers in microbiology · 2021Article
- Clp is a "busy" transcription factor in the bacterial warrior,Computational and structural biotechnology journal · 2021Review
- ChIP-seq Analysis of the Global Regulator Vfr Reveals Novel Insights Into the Biocontrol AgentFrontiers in microbiology · 2021Article
- An intrinsic mechanism for coordinated production of the contact-dependent and contact-independent weapon systems in a soil bacterium.PLoS pathogens · 2020Article
- LeTetR Positively Regulates 3-Hydroxylation of the Antifungal HSAF and Its Analogs inMolecules (Basel, Switzerland) · 2020Article
- Two Functional Fatty Acyl Coenzyme A Ligases Affect Free Fatty Acid Metabolism To Block Biosynthesis of an Antifungal Antibiotic in Lysobacter enzymogenes.Applied and environmental microbiology · 2020Article
- A YajQ-LysR-like, cyclic di-GMP-dependent system regulating biosynthesis of an antifungal antibiotic in a crop-protecting bacterium, Lysobacter enzymogenes.Molecular plant pathology · 2020Article
- Interspecies and Intraspecies Signals Synergistically Regulate Lysobacter enzymogenes Twitching Motility.Applied and environmental microbiology · 2019Article
- Two forms of phosphomannomutase in gammaproteobacteria: The overlooked membrane-bound form of AlgC is required for twitching motility of Lysobacter enzymogenes.Environmental microbiology · 2019Article
- Systematic optimization for production of the anti-MRSA antibiotics WAP-8294A in an engineered strain of Lysobacter enzymogenes.Microbial biotechnology · 2019Article
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
Lysobacter enzymogenes is a bacterial biological control agent emerging as a new source of antibiotic metabolites, such as heat-stable antifungal factor (HSAF) and the antibacterial factor WAP-8294A2. The regulatory mechanism(s) for antibiotic metabolite biosynthesis remains largely unknown in L. enzymogenes. Clp, a cyclic adenosine monophosphate (cAMP)-receptor-like protein, is shown to function as a global regulator in modulating biocontrol-associated traits in L. enzymogenes. However, the genetic basis of Clp signaling remains unclear. Here, we utilized transcriptome/microarray analysis to determine the Clp regulon in L. enzymogenes. We showed that Clp is a global regulator in gene expression, as the transcription of 775 genes belonging to 19 functional groups was differentially controlled by Clp signaling. Analysis of the Clp regulon detected previously characterized Clp-modulated functions as well as novel loci. These include novel loci involved in antibiotic metabolite biosynthesis and surface motility in L. enzymogenes. We further showed experimentally that Clp signaling played a positive role in regulating the biosynthesis of HSAF and WAP-8294A2, as well as surface motility which is a type-IV-pilus-dependent trait. The regulation by Clp signaling of antibiotic (HSAF and WAP-8294A2) biosynthesis and surface motility was found to be independent. Importantly, we identified a factor Lysobacter acetyltransferase (Lat), a homologue of histone acetyltransferase Hpa2, which was regulated by Clp and involved in HSAF biosynthesis, but not associated with WAP-8294A2 production and surface motility. Overall, our study provided new insights into the regulatory role and molecular mechanism of Clp signaling in L. enzymogenes.
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