ArticleAnalytical chemistry2014
Subsecond electrophoretic separations from droplet samples for screening of enzyme modulators.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- On-Chip Photonic Detection Techniques for Non-Invasive In Situ Characterizations at the Microfluidic Scale.Sensors (Basel, Switzerland) · 2024Review
- Comparison of Design Approaches for Low-Cost Sampling Mechanisms in Open-Source Chemical Instrumentation.HardwareX · 2021Article
- Active Flow Control and Dynamic Analysis in Droplet Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Review
- Two Orders of Magnitude Boost in the Detection Limit of Droplet-Based Micro-Magnetofluidics with Planar Hall Effect Sensors.ACS omega · 2020Article
- Droplet sample introduction to microchip gel and zone electrophoresis for rapid analysis of protein-protein complexes and enzymatic reactions.Analytical and bioanalytical chemistry · 2019Article
- Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays.Molecules (Basel, Switzerland) · 2019Review
- Droplet Microfluidics in Thermoplastics: Device Fabrication, Droplet Generation, and Content Manipulation using Integrated Electric and Magnetic Fields.Analytical methods : advancing methods and applications · 2018Article
- In Vivo Chemical Monitoring at High Spatiotemporal Resolution Using Microfabricated Sampling Probes and Droplet-Based Microfluidics Coupled to Mass Spectrometry.Analytical chemistry · 2018Article
- Integrated, Continuous Emulsion Creamer.Analytical chemistry · 2017Article
- Advances in capillary electrophoresis and the implications for drug discovery.Expert opinion on drug discovery · 2017Review
- Automatic Combination of Microfluidic Nanoliter-Scale Droplet Array with High-Speed Capillary Electrophoresis.Scientific reports · 2016Article
- Discovery in Droplets.Analytical chemistry · 2016Review
- Identification of sirtuin 5 inhibitors by ultrafast microchip electrophoresis using nanoliter volume samples.Analytical and bioanalytical chemistry · 2016Article
- Capillary zone electrophoresis as a tool for bottom-up protein analysis.Bioanalysis · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
High-throughput screening (HTS) using multiwell plates and fluorescence plate readers is a powerful tool for drug discovery and evaluation by allowing tens of thousands of assays to be completed in 1 day. Although this method has been successful, electrophoresis-based methods for screening are also of interest to avoid difficulties associated fluorescence assays such as requirements to engineer fluorogenic reactions and false positives. We have developed a method using droplet microfluidics to couple multiwell plate-based assays to microchip electrophoresis (MCE) to screen enzyme modulators. Samples contained in multiwell plates are reformatted in to plugs with a sample volume of 8 nL segmented by an immiscible oil. The segmented flow sample streams are coupled to a hybrid polydimethylsiloxane-glass microfluidic device capable of selectively extracting the aqueous samples from the droplet stream and rapidly analyzing by MCE with laser-induced fluorescence detection. This system was demonstrated by screening a test library of 140 compounds against using protein kinase A. For each sample in the screen, two droplets are generated, allowing approximately 6 MCE injections per sample. Using a 1 s separation at 2000 V/cm, we are able to analyze 96 samples in 12 min. Separation resolution between the internal standard, substrate, and product is 1.2 and average separation efficiency is 16,000 plates/s using real samples. Twenty-five compounds were identified as modulators during primary screening and verified using dose-response curves.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.