ArticleeLife2014
Discovery of a small molecule that inhibits bacterial ribosome biogenesis.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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Who cites it
54 citing papers in PubMed, 91 citations in OpenAlex.
- Psychotropic Drugs: A Promising Non-Antibiotic Agent to Combat Multi-Drug-Resistant Microorganisms.Drug development research · 2026Review
- Analysis of treatment-naïve gut microbiota in children with new-onset epilepsy.Pediatric research · 2026Article
- A complex between IF2 and NusA suggests early coupling of transcription-translation.Nature communications · 2025Article
- Coupling of ribosome biogenesis and translation initiation in human mitochondria.Nature communications · 2025Article
- Role of the sarcin-ricin loop of 23S rRNA in biogenesis of the 50S ribosomal subunit.RNA (New York, N.Y.) · 2025Article
- Bacterial IF2's N-terminal IDR drives cold-induced phase separation and promotes fitness during cold stress.bioRxiv : the preprint server for biology · 2025Article
- The proline-rich antimicrobial peptide Api137 disrupts large ribosomal subunit assembly and induces misfolding.Nature communications · 2025Article
- Article
- Lamotrigine-mediated rescue of RsgA-deficientJournal of bacteriology · 2024Article
- Antibacterial activity of nonantibiotics is orthogonal to standard antibiotics.Science (New York, N.Y.) · 2024Article
- The Role of the Vagus Nerve in the Microbiome and Digestive System in Relation to Epilepsy.Current medicinal chemistry · 2024Review
- Synthesis of vancomycin fluorescent probes that retain antimicrobial activity, identify Gram-positive bacteria, and detect Gram-negative outer membrane damage.Communications biology · 2023Article
- Near-physiological in vitro assembly of 50S ribosomes involves parallel pathways.Nucleic acids research · 2023Article
- Topiramate alters the gut microbiome to aid in its anti-seizure effect.Frontiers in microbiology · 2023Article
- Lamotrigine compromises the fidelity of initiator tRNA recruitment to the ribosomal P-site by IF2 and the RbfA release from 30S ribosomes inRNA biology · 2023Article
- Quantitative mining of compositional heterogeneity in cryo-EM datasets of ribosome assembly intermediates.Structure (London, England : 1993) · 2022Article
- The effect of a novel anticonvulsant chemical Q808 on gut microbiota and hippocampus neurotransmitters in pentylenetetrazole-induced seizures in rats.BMC neuroscience · 2022Article
- The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation.RNA biology · 2022Article
- Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing.Frontiers in nutrition · 2022Article
- Changes and significance of gut microbiota in children with focal epilepsy before and after treatment.Frontiers in cellular and infection microbiology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
While small molecule inhibitors of the bacterial ribosome have been instrumental in understanding protein translation, no such probes exist to study ribosome biogenesis. We screened a diverse chemical collection that included previously approved drugs for compounds that induced cold sensitive growth inhibition in the model bacterium Escherichia coli. Among the most cold sensitive was lamotrigine, an anticonvulsant drug. Lamotrigine treatment resulted in the rapid accumulation of immature 30S and 50S ribosomal subunits at 15 °C. Importantly, this was not the result of translation inhibition, as lamotrigine was incapable of perturbing protein synthesis in vivo or in vitro. Spontaneous suppressor mutations blocking lamotrigine activity mapped solely to the poorly characterized domain II of translation initiation factor IF2 and prevented the binding of lamotrigine to IF2 in vitro. This work establishes lamotrigine as a widely available chemical probe of bacterial ribosome biogenesis and suggests a role for E. coli IF2 in ribosome assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.