Evidence map›Paper›PMID 25232648›Full record

ArticleAnalytical chemistry2014

Standing surface acoustic wave based cell coculture.

Sixing Li, Feng Guo, Yuchao Chen, Xiaoyun Ding, Peng Li, Lin Wang, Craig E Cameron, Tony Jun Huang

Open access · greenAbstract read
In one paragraph

Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 97 citations in OpenAlex.

  1. Review
  2. Acoustic Waves Coupling with Polydimethylsiloxane in Reconfigurable Acoustofluidic Platform.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Review
  8. Article
  9. Acoustofluidic Engineering of Functional Vessel-on-a-Chip.ACS biomaterials science & engineering · 2023
    Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Sonoporation: Past, Present, and Future.Advanced materials technologies · 2022
    Article
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Sixing LiDepartment of Engineering Science and Mechanics, The Pennsylvania State University , University Park, Pennsylvania 16802, United States.
Feng Guo
Yuchao Chen
Xiaoyun Ding
Peng Li
Lin Wang
Craig E Cameron
Tony Jun Huang
Pennsylvania State University · USAscent Bio-Nano Technologies (United States) · US

Funding

RNA DEPENDENT RNA POLYMERASE MECHANISMR01AI045818 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ARNOLD, JAMIE JON, CAMERON, CRAIG E. · 1999 to 2025
$7.5M
On-Chip Optofluidic Laser Scanning Confocal Microscope for Early Cancer DetectionDP2OD007209 · OD · PENNSYLVANIA STATE UNIVERSITY, THE · PI HUANG, TONY JUN · 2010 to 2010
$2.2M
Standing Surface Acoustic Wave Based Cell Sorters for Maintaining Cell IntegrityR01GM112048 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI HUANG, TONY JUN · 2014 to 2017
$1.1M
NIAID NIH HHS AI45818NIAID NIH HHS R01 AI045818NIGMS NIH HHS 1 R01 GM112048-01A1NIGMS NIH HHS R01 GM112048NIH HHS 1DP2OD007209-01NIH HHS DP2 OD007209
6 · The paper itself

Abstract

Precise reconstruction of heterotypic cell-cell interactions in vitro requires the coculture of different cell types in a highly controlled manner. In this article, we report a standing surface acoustic wave (SSAW)-based cell coculture platform. In our approach, different types of cells are patterned sequentially in the SSAW field to form an organized cell coculture. To validate our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells. Real-time monitoring of cell migration dynamics reveals increased cancer cell mobility when cancer cells are cocultured with endothelial cells. Our SSAW-based cell coculture platform has the advantages of contactless cell manipulation, high biocompatibility, high controllability, simplicity, and minimal interference of the cellular microenvironment. The SSAW technique demonstrated here can be a valuable analytical tool for various biological studies involving heterotypic cell-cell interactions.

Indexed as

Microfluidic Analytical TechniquesSoundCell CommunicationCell MovementCoculture TechniquesEndothelial CellsEquipment DesignFluoresceinsFluorescent DyesHeLa CellsHumansTime-Lapse Imaging5-chloromethylfluoresceincalcein AMFluoresceinsFluorescent Dyes

Identifiers

PMID25232648
PMCPMC4188268
OpenAlexW2335218714

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.