ArticleNature communications2014
In vivo single-molecule imaging identifies altered dynamics of calcium channels in dystrophin-mutant C. elegans.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- The nanoscale mobility of calcium channels is driven by readily releasable synaptic vesicles to support precise neurotransmission in livebioRxiv : the preprint server for biology · 2026Article
- Single-Molecule Localization Microscopy in vivo at the Drosophila Neuromuscular Junction.Methods in molecular biology (Clifton, N.J.) · 2026Article
- SlimVar for rapid in vivo single-molecule tracking of chromatin regulators in plants.Nature communications · 2025Article
- Treasure on the Earth-Gold Nanoparticles and Their Biomedical Applications.Materials (Basel, Switzerland) · 2022Review
- Dynamic motions of ice-binding proteins in livingBiochemistry and biophysics reports · 2022Article
- Calcium handling maturation and adaptation to increased substrate stiffness in human iPSC-derived cardiomyocytes: The impact of full-length dystrophin deficiency.Frontiers in physiology · 2022Article
- Animal models for researching approaches to therapy of Duchenne muscular dystrophy.Transgenic research · 2021Review
- Review
- Mitochondrial hydrogen sulfide supplementation improves health in theProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Defects of full-length dystrophin trigger retinal neuron damage and synapse alterations by disrupting functional autophagy.Cellular and molecular life sciences : CMLS · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- "Betwixt Mine Eye and Heart a League Is Took": The Progress of Induced Pluripotent Stem-Cell-Based Models of Dystrophin-Associated Cardiomyopathy.International journal of molecular sciences · 2020Review
- Absence of full-length dystrophin impairs normal maturation and contraction of cardiomyocytes derived from human-induced pluripotent stem cells.Cardiovascular research · 2020Article
- Advances in Stem Cell Modeling of Dystrophin-Associated Disease: Implications for the Wider World of Dilated Cardiomyopathy.Frontiers in physiology · 2020Review
- Brief update on endocytosis of nanomedicines.Advanced drug delivery reviews · 2019Review
- Single-molecule imaging correlates decreasing nuclear volume with increasing TF-chromatin associations during zebrafish development.Nature communications · 2018Article
- Muscle strength deficiency and mitochondrial dysfunction in a muscular dystrophy model ofDisease models & mechanisms · 2018Article
- α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Characterization of Split Fluorescent Protein Variants and Quantitative Analyses of Their Self-Assembly Process.Scientific reports · 2018Article
- Cellular imaging by targeted assembly of hot-spot SERS and photoacoustic nanoprobes using split-fluorescent protein scaffolds.Nature communications · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
Single-molecule (SM) fluorescence microscopy allows the imaging of biomolecules in cultured cells with a precision of a few nanometres but has yet to be implemented in living adult animals. Here we used split-GFP (green fluorescent protein) fusions and complementation-activated light microscopy (CALM) for subresolution imaging of individual membrane proteins in live Caenorhabditis elegans (C. elegans). In vivo tissue-specific SM tracking of transmembrane CD4 and voltage-dependent Ca(2+) channels (VDCC) was achieved with a precision of 30 nm within neuromuscular synapses and at the surface of muscle cells in normal and dystrophin-mutant worms. Through diffusion analyses, we reveal that dystrophin is involved in modulating the confinement of VDCC within sarcolemmal membrane nanodomains in response to varying tonus of C. elegans body-wall muscles. CALM expands the applications of SM imaging techniques beyond the petri dish and opens the possibility to explore the molecular basis of homeostatic and pathological cellular processes with subresolution precision, directly in live animals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.