ArticleFrontiers in neuroanatomy2014
Long-term Cre-mediated retrograde tagging of neurons using a novel recombinant pseudorabies virus.
Article in Frontiers in neuroanatomy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Preparation and application of YFV-17D-derived Anterograde Trans-neuronal Viral Vectors for Neuroscience Research.bioRxiv : the preprint server for biology · 2026Article
- Advances in the labelling and selective manipulation of synapses.Nature reviews. Neuroscience · 2024Review
- Virus-Based Neural Circuit Tracing.Advances in neurobiology · 2024Review
- Transneuronal Circuit Analysis with Pseudorabies Viruses.Current protocols · 2023Review
- Directed stepwise tracing of polysynaptic neuronal circuits with replication-deficient pseudorabies virus.Cell reports methods · 2023Article
- Viral Tools for Neural Circuit Tracing.Neuroscience bulletin · 2022Review
- Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D.Nature methods · 2021Article
- Activity of the Lateral Hypothalamus during Genetically Determined Absence Seizures.International journal of molecular sciences · 2021Article
- Can transsynaptic viral strategies be used to reveal functional aspects of neural circuitry?Journal of neuroscience methods · 2021Review
- Viral tools for neuroscience.Nature reviews. Neuroscience · 2020Review
- Neuroanatomical tract-tracing techniques that did go viral.Brain structure & function · 2020Review
- Pseudorabies Virus Infection Accelerates Degradation of the Kinesin-3 Motor KIF1A.Journal of virology · 2020Article
- Trans-synaptic Neural Circuit-Tracing with Neurotropic Viruses.Neuroscience bulletin · 2019Review
- Hitchhiking on the neuronal highway: Mechanisms of transsynaptic specificity.Journal of chemical neuroanatomy · 2019Review
- Differences in neurotropism and neurotoxicity among retrograde viral tracers.Molecular neurodegeneration · 2019Article
- A Student's Guide to Neural Circuit Tracing.Frontiers in neuroscience · 2019Review
- Development of an optogenetic toolkit for neural circuit dissection in squirrel monkeys.Scientific reports · 2018Article
- Review
- Article
- Nonhuman Primate Optogenetics: Recent Advances and Future Directions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Review
Corrections and comments
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Authors and funding
5 authors at 5 institutions in 2 countries.
Funding
Abstract
Brain regions contain diverse populations of neurons that project to different long-range targets. The study of these subpopulations in circuit function and behavior requires a toolkit to characterize and manipulate their activity in vivo. We have developed a novel set of reagents based on Pseudorabies Virus (PRV) for efficient and long-term genetic tagging of neurons based on their projection targets. By deleting IE180, the master transcriptional regulator in the PRV genome, we have produced a mutant virus capable of infection and transgene expression in neurons but unable to replicate in or spread from those neurons. IE180-null mutants showed no cytotoxicity, and infected neurons exhibited normal physiological function more than 45 days after infection, indicating the utility of these engineered viruses for chronic experiments. To enable rapid and convenient construction of novel IE180-null recombinants, we engineered a bacterial artificial chromosome (BAC) shuttle-vector system for moving new constructs into the PRV IE180-null genome. Using this system we generated an IE180-null recombinant virus expressing the site-specific recombinase Cre. This Cre-expressing virus (PRV-hSyn-Cre) efficiently and robustly infects neurons in vivo and activates transgene expression from Cre-dependent vectors in local and retrograde projecting populations of neurons in the mouse. We also generated an assortment of recombinant viruses expressing fluorescent proteins (mCherry, EGFP, ECFP). These viruses exhibit long-term labeling of neurons in vitro but transient labeling in vivo. Together these novel IE180-null PRV reagents expand the toolkit for targeted gene expression in the brain, facilitating functional dissection of neuronal circuits in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.