Evidence map›Paper›PMID 25232168›Full record

ArticleJournal of clinical microbiology2014

Evaluation of high-resolution melting curve analysis of ligation-mediated real-time PCR, a rapid method for epidemiological typing of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter Species) pathogens.

Hanna Woksepp, Anna Ryberg, Hanna Billström, Anita Hällgren, Lennart E Nilsson, Britt-Inger Marklund, Barbro Olsson-Liljequist, Thomas Schön

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
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  6. Article
  7. Rapid Methods for Antimicrobial Resistance Diagnostics.Antibiotics (Basel, Switzerland) · 2021
    Review
  8. Article
  9. Article
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  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hanna WokseppDepartment of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden Department of Medicine and Optometry, Linnaeus University, Kalmar, Sweden hanna.woksepp@ltkalmar.se.
Anna RybergDepartment of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Hanna BillströmThe Public Health Agency of Sweden, Solna, Sweden.
Anita HällgrenDepartment of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
Lennart E NilssonDepartment of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.
Britt-Inger MarklundDepartment of Chemistry and Biomedical Sciences, Linnaeus University, Kalmar, Sweden.
Barbro Olsson-LiljequistThe Public Health Agency of Sweden, Solna, Sweden.
Thomas SchönDepartment of Clinical Microbiology, Kalmar County Hospital, Kalmar, Sweden Department of Medicine and Optometry, Linnaeus University, Kalmar, Sweden Department of Clinical Microbiology and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A single-tube method, ligation-mediated real-time PCR high-resolution melt analysis (LMqPCR HRMA), was modified for the rapid typing of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE) pathogens. A 97% agreement (60/62 isolates) was achieved in comparison to pulsed-field gel electrophoresis (PFGE) results, which indicates that LMqPCR HRMA is a rapid and accurate screening tool for monitoring nosocomial outbreaks.

Indexed as

Bacterial Typing TechniquesCross InfectionDNA, BacterialGram-Negative BacteriaGram-Negative Bacterial InfectionsGram-Positive BacteriaGram-Positive Bacterial InfectionsHumansMolecular EpidemiologyReal-Time Polymerase Chain ReactionTime FactorsTransition TemperatureDNA, Bacterial

Identifiers

PMID25232168
PMCPMC4313309

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.