ArticleProceedings of the National Academy of Sciences of the United States of America2014
Silencing of a metaphase I-specific gene results in a phenotype similar to that of the Pairing homeologous 1 (Ph1) gene mutations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- An overview of recent advances on wheat homologous and homoeologous recombination.Journal of experimental botany · 2026Review
- Stable resynthesized Brassica napus lines show similar meiotic behaviour to established B. napus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Article
- Origin of small chromosome A08 and genome evolution of Arachis species.Nature communications · 2026Article
- Hybridization between different Brassica allohexaploid lineages produces viable, fertile progeny but does not improve genome stability or fertility in F1 hybrids.Annals of botany · 2026Article
- Development and Characterization of Wheat-Plants (Basel, Switzerland) · 2025Article
- A Bread Wheat Line with the Substituted Wild Emmer Chromosome 4A Results in Fragment Deletions of Chromosome 4B and Weak Plants.Plants (Basel, Switzerland) · 2025Article
- Defining autopolyploidy: Cytology, genetics, and taxonomy.American journal of botany · 2024Review
- Mapping heat tolerance QTLs inFrontiers in plant science · 2024Article
- Rapid development of wheat-Dasypyrum villosum compensating translocations resistant to powdery mildew using a triple marker strategy conducted on a large ph1b-induced population.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Evolution and origin of bread wheat.The Plant cell · 2022Article
- Knock-down of gene expression throughout meiosis and pollen formation by virus-induced gene silencing in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Tracing the Evolution of the Angiosperm Genome from the Cytogenetic Point of View.Plants (Basel, Switzerland) · 2022Review
- Integrated cytological and transcriptomic analysis reveals insights into pollen fertility in newly syntheticFrontiers in plant science · 2022Article
- Variations of wheat (Frontiers in plant science · 2022Article
- Dissection of Structural Reorganization of Wheat 5B Chromosome Associated With Interspecies Recombination Suppression.Frontiers in plant science · 2022Article
- Review
- Challenges and prospects for a potential allohexaploid Brassica crop.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Review
- Meiotic crossover reduction by virus-induced gene silencing enables the efficient generation of chromosome substitution lines and reverse breeding in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Chromosome Pairing in Polyploid Grasses.Frontiers in plant science · 2020Review
- Homoeologous Exchanges, Segmental Allopolyploidy, and Polyploid Genome Evolution.Frontiers in genetics · 2020Review
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Although studied extensively since 1958, the molecular mode of action of the Pairing homeologous 1 (Ph1) gene is still unknown. In polyploid wheat, the diploid-like chromosome pairing is principally controlled by the Ph1 gene via preventing homeologous chromosome pairing (HECP). Here, we report a candidate Ph1 gene (C-Ph1) present in the Ph1 locus, transient as well as stable silencing of which resulted in a phenotype characteristic of the Ph1 gene mutants, including HECP, multivalent formation, and disrupted chromosome alignment on the metaphase I (MI) plate. Despite a highly conserved DNA sequence, the C-Ph1 gene homeologues showed a dramatically different structure and expression pattern, with only the 5B copy showing MI-specific expression, further supporting our claim for the Ph1 gene. In agreement with the previous reports about the Ph1 gene, the predicted protein of the 5A copy of the C-Ph1 gene is truncated, and thus perhaps less effective. The 5D copy is expressed around the onset of meiosis; thus, it may function during the earlier stages of chromosome pairing. Along with alternate splicing, the predicted protein of the 5B copy is different from the protein of the other two copies because of an insertion. These structural and expression differences among the homeologues concurred with the previous observations about Ph1 gene function. Stable RNAi silencing of the wheat gene in Arabidopsis showed multivalents and centromere clustering during meiosis I.
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