ArticleMolecular biology of the cell2014
Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
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- Production of CRISPR-Cas9 Transgenic Cell Lines for Knocksideways Studies.Current protocols · 2023Article
- Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA.Open biology · 2022Article
- cNap1 bridges centriole contact sites to maintain centrosome cohesion.PLoS biology · 2022Article
- Tip60 acetylation of histone H3K4 temporally controls chromosome passenger complex localization.Molecular biology of the cell · 2022Article
- Correlative nanophotonic approaches to enlighten the nanoscale dynamics of living cell membranes.Biochemical Society transactions · 2021Review
- Three-dimensional superresolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex.Molecular biology of the cell · 2021Article
- Article
- A Condensed View of the Chromosome Passenger Complex.Trends in cell biology · 2020Review
- Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs.Stem cell reports · 2019Article
- The binding of Borealin to microtubules underlies a tension independent kinetochore-microtubule error correction pathway.Nature communications · 2019Article
- Efficient genome editing using CRISPR-Cas-mediated homology directed repair in the ascidian Ciona robusta.Genesis (New York, N.Y. : 2000) · 2018Article
- A quantitative map of human Condensins provides new insights into mitotic chromosome architecture.The Journal of cell biology · 2018Article
- Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four-dimensional imaging.Nature protocols · 2018Article
- Generation and validation of homozygous fluorescent knock-in cells using CRISPR-Cas9 genome editing.Nature protocols · 2018Article
- Insight into the fission mechanism by quantitative characterization of Drp1 protein distribution in the living cell.Scientific reports · 2018Article
- Article
- Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time.Scientific reports · 2017Article
- A proposal for validation of antibodies.Nature methods · 2016Article
Corrections and comments
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8 authors.
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Abstract
Fluorescence tagging of proteins is a widely used tool to study protein function and dynamics in live cells. However, the extent to which different mammalian transgene methods faithfully report on the properties of endogenous proteins has not been studied comparatively. Here we use quantitative live-cell imaging and single-molecule spectroscopy to analyze how different transgene systems affect imaging of the functional properties of the mitotic kinase Aurora B. We show that the transgene method fundamentally influences level and variability of expression and can severely compromise the ability to report on endogenous binding and localization parameters, providing a guide for quantitative imaging studies in mammalian cells.
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