ArticleDevelopment (Cambridge, England)2014
Translational profiling through biotinylation of tagged ribosomes in zebrafish.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Rapgef1 paralog-mediated regulation of Wnt/β-catenin signaling orchestrates early embryo tissue patterning and morphogenesis.Cell communication and signaling : CCS · 2026Article
- CaOpen biology · 2025Article
- In vivo profiling of the endothelium using 'AngioTag' zebrafish.Angiogenesis · 2025Article
- CXCR3-CXCL11 Signaling Restricts Angiogenesis and Promotes Pericyte Recruitment.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Transglutaminase Type 2 regulates the Wnt/β-catenin pathway in vertebrates.Cell death & disease · 2021Article
- In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish.eLife · 2021Article
- Article
- Trap-TRAP, a Versatile Tool for Tissue-Specific Translatomics in Zebrafish.Frontiers in cell and developmental biology · 2021Article
- Human cytomegaloviral multifunctional protein kinase pUL97 impairs zebrafish embryonic development and increases mortality.Scientific reports · 2019Article
- The codon sequences predict protein lifetimes and other parameters of the protein life cycle in the mouse brain.Scientific reports · 2018Article
- Robust Identification of Developmentally Active Endothelial Enhancers in Zebrafish Using FANS-Assisted ATAC-Seq.Cell reports · 2017Article
- Biotagging of Specific Cell Populations in Zebrafish Reveals Gene Regulatory Logic Encoded in the Nuclear Transcriptome.Cell reports · 2017Article
- Cavin4b/Murcb Is Required for Skeletal Muscle Development and Function in Zebrafish.PLoS genetics · 2016Article
- Function Over Form: Modeling Groups of Inherited Neurological Conditions in Zebrafish.Frontiers in molecular neuroscience · 2016Review
- Ribosome profiling reveals the what, when, where and how of protein synthesis.Nature reviews. Molecular cell biology · 2015Review
- Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafish.BMC genomics · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterogeneity within a population of cells of the same type is a common theme in metazoan biology. Dissecting complex developmental and physiological processes crucially relies on our ability to probe the expression profile of these cell subpopulations. Current strategies rely on cell enrichment based on sequential or simultaneous use of multiple intersecting markers starting from a heterogeneous cell suspension. The extensive tissue manipulations required to generate single-cell suspensions, as well as the complexity of the required equipment, inherently complicate these approaches. Here, we propose an alternative methodology based on a genetically encoded system in the model organism Danio rerio (zebrafish). In transgenic fish, we take advantage of the combinatorial biotin transfer system, where polysome-associated mRNAs are selectively recovered from cells expressing both a tagged ribosomal subunit, Rpl10a, and the bacterial biotin ligase BirA. We have applied this technique to skeletal muscle development and identified new genes with interesting temporal expression patterns. Through this work we have thus developed additional tools for highly specific gene expression profiling.
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