ArticleCytotechnology2015
Migration and differentiation of transplanted enteric neural crest-derived cells in murine model of Hirschsprung's disease.
Article in Cytotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The trial behind it
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Enteric nervous system: sensory transduction, neural circuits and gastrointestinal motility.Nature reviews. Gastroenterology & hepatology · 2020Review
- Isolation and Characterization of Neural Crest-Derived Stem Cells From Adult Ovine Palatal Tissue.Frontiers in cell and developmental biology · 2018Article
- Transplantation of enteric nervous system stem cells rescues nitric oxide synthase deficient mouse colon.Nature communications · 2017Article
- Exposure to GDNF Enhances the Ability of Enteric Neural Progenitors to Generate an Enteric Nervous System.Stem cell reports · 2017Article
- New approaches to increase intestinal length: Methods used for intestinal regeneration and bioengineering.World journal of transplantation · 2016Review
- Fluorescence Visualization of the Enteric Nervous Network in a Chemically Induced Aganglionosis Model.PloS one · 2016Article
- In Vivo Transplantation of Enteric Neural Crest Cells into Mouse Gut; Engraftment, Functional Integration and Long-Term Safety.PloS one · 2016Article
- Surgical Intervention to Rescue Hirschsprung Disease in a Rat Model.Journal of neurogastroenterology and motility · 2015Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cell therapy offers the potential of rebuilding the enteric nervous system (ENS) in the aganglionic bowel of patients with Hirschsprung's disease. P0-Cre/Floxed-EGFP mice in which neural crest-derived cells express EGFP were used to obtain ENS stem/progenitor cells. ENS stem/progenitor cells were transplanted into the bowel of Ret(-/-) mouse, an animal model of Hirschsprung's disease. Immunohistochemical analysis was performed to determine whether grafted cells gave rise to neurons in the recipient bowel. EGFP expressing neural crest-derived cells accounted for 7.01 ± 2.52 % of total cells of gastrointestinal tract. ENS stem/progenitor cells were isolated using flow cytometry and expanded as neurosphere-like bodies (NLBs) in a serum-free culture condition. Some cells in NLBs expressed neural crest markers, p75 and Sox10 and neural stem/progenitor cells markers, Nestin and Musashi1. Multipotency of isolated ENS stem/progenitor cells was determined as they differentiated into neurons, glial cells, and myofibloblasts in culture. When co-cultured with explants of hindgut of Ret(-/-) mice, ENS stem/progenitor cells migrated into the aganglionic bowel and gave rise to neurons. ENS stem/progenitor cells used in this study appear to be clinically relevant donor cells in cell therapy to treat Hirschsprung's disease capable of colonizing the affected bowel and giving rise to neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.