ArticleRevista do Instituto de Medicina Tropical de Sao Paulo
Detection of virulence genes in environmental strains of Vibrio cholerae from estuaries in northeastern Brazil.
Article in Revista do Instituto de Medicina Tropical de Sao Paulo. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Genomic characterization of clinical and environmentalFrontiers in microbiology · 2026Article
- Molecular characterization of Vibrio species isolated from dairy and water samples.Scientific reports · 2023Article
- Virulence genes and antibiotic resistance profile ofVeterinary research forum : an international quarterly journal · 2022Article
- Virulence and Antibiotic Resistance Characteristics ofFrontiers in cellular and infection microbiology · 2021Article
- Molecular Detection and Distribution of Six Medically ImportantFrontiers in microbiology · 2021Article
- Virulence Pattern and Genomic Diversity of Vibrio cholerae O1 and O139 Strains Isolated From Clinical and Environmental Sources in India.Frontiers in microbiology · 2020Article
- Characterisation ofInternational journal of microbiology · 2020Article
- Characterization of highly virulent multidrug resistant Vibrio cholerae isolated from a large cholera outbreak in Ghana.BMC research notes · 2018Article
- Limpet (Frontiers in public health · 2018Article
- Virulence factor activity relationships (VFARs): a bioinformatics perspective.Environmental science. Processes & impacts · 2017Review
- Riverbed Sediments as Reservoirs of MultipleJournal of environmental and public health · 2017Article
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Authors and funding
8 authors.
Funding
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Abstract
The objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological resemblance to V. cholerae were isolated. The cultures were identified phenotypically using a dichotomous key based on biochemical characteristics. The total DNA extracted was amplified by PCR to detect ompW and by multiplex PCR to detect the virulence genes ctx, tcp, zot and rfbO1. The results of the phenotypic and genotypic identification were compared. Nine strains of V. cholerae were identified phenotypically, five of which were confirmed by detection of the species-specific gene ompW. The dichotomous key was efficient at differentiating environmental strains of V. cholerae. Strains of V. cholerae were found in all four estuaries, but none possessed virulence genes.
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