ArticleBiophysical journal2014
Calibration of optical tweezers for in vivo force measurements: how do different approaches compare?
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Who cites it
43 citing papers in PubMed.
- Optical Tweezers in Emulsion Research: Principles, Advances, and Prospects.Langmuir : the ACS journal of surfaces and colloids · 2026Review
- Planar lightsheet optical tweezer pLOT for 2D trapping and imaging of freely-moving live cells.Communications biology · 2025Article
- FAIR data for optical tweezers experiments.Biophysical journal · 2025Review
- Measuring age-dependent viscoelasticity of organelles, cells and organisms with time-shared optical tweezer microrheology.Nature nanotechnology · 2025Article
- Multiple kinesins speed up cargo transport in crowded environments by sharing load.Communications biology · 2025Article
- Intracellular Macromolecular Crowding within Individual Stress Fibers Analyzed by Fluorescence Correlation Spectroscopy.Cellular and molecular bioengineering · 2024Article
- Life under tension: the relevance of force on biological polymers.Biophysics reports · 2024Article
- Optical Tweezers Apparatus Based on a Cost-Effective IR Laser-Hardware and Software Description.Sensors (Basel, Switzerland) · 2024Article
- Studying fluctuating trajectories of optically confined passive tracers inside cells provides familiar active forces.Biomedical optics express · 2023Article
- Bacterial cellulose: Nano-biomaterial for biodegradable face masks - A greener approach towards environment.Environmental nanotechnology, monitoring & management · 2023Review
- Wavelength-Tunable Narrow-Linewidth Laser Diode Based on Self-Injection Locking with a High-Q Lithium Niobate Microring Resonator.Nanomaterials (Basel, Switzerland) · 2023Article
- Unravelling 3D Dynamics and Hydrodynamics during Incorporation of Dielectric Particles to an Optical Trapping Site.ACS nano · 2023Article
- Looking at Biomolecular Interactions through the Lens of Correlated Fluorescence Microscopy and Optical Tweezers.International journal of molecular sciences · 2023Review
- Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers.Frontiers in physics · 2023Article
- Surface Modification of Bacterial Cellulose for Biomedical Applications.International journal of molecular sciences · 2022Review
- Programmable viscoelasticity in protein-RNA condensates with disordered sticker-spacer polypeptides.Nature communications · 2021Article
- Lateral Deformation of Human Red Blood Cells by Optical Tweezers.Micromachines · 2021Article
- Temperature Effects on Optical Trapping Stability.Micromachines · 2021Review
- Multi-frequency passive and active microrheology with optical tweezers.Scientific reports · 2021Article
- Optical manipulation: advances for biophotonics in the 21st century.Journal of biomedical optics · 2021Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
There is significant interest in quantifying force production inside cells, but since conditions in vivo are less well controlled than those in vitro, in vivo measurements are challenging. In particular, the in vivo environment may vary locally as far as its optical properties, and the organelles manipulated by the optical trap frequently vary in size and shape. Several methods have been proposed to overcome these difficulties. We evaluate the relative merits of these methods and directly compare two of them, a refractive index matching method, and a light-momentum-change method. Since in vivo forces are frequently relatively high (e.g., can exceed 15 pN for lipid droplets), a high-power laser is employed. We discover that this high-powered trap induces local temperature changes, and we develop an approach to compensate for uncertainties in the magnitude of applied force due to such temperature variations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.