ArticleBiophysical journal2014
Imaging fast calcium currents beyond the limitations of electrode techniques.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Neuronal Imaging at 8-Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz.Journal of biophotonics · 2025Article
- Fast Neuronal Calcium Signals in Brain Slices Loaded With Fluo-4 AM Ester.The European journal of neuroscience · 2025Article
- Analysis of the effect of the scorpion toxin AaH-II on action potential generation in the axon initial segment.Scientific reports · 2024Article
- Spatial and temporal aspects of neuronal calcium and sodium signals measured with low-affinity fluorescent indicators.Pflugers Archiv : European journal of physiology · 2024Review
- Kinetics and functional consequences of BK channels activation by N-type CaFrontiers in cellular neuroscience · 2024Article
- Can neuron modeling constrained by ultrafast imaging data extract the native function of ion channels?Frontiers in computational neuroscience · 2023Article
- In vivo spatiotemporal control of voltage-gated ion channels by using photoactivatable peptidic toxins.Nature communications · 2022Article
- Cal-520FF is the Present Optimal CaJournal of fluorescence · 2021Article
- CaeLife · 2020Article
- The Origin of Physiological Local mGluR1 Supralinear CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Two Distinct Sets of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Editorial: New Insights on Neuron and Astrocyte Function From Cutting-Edge Optical Techniques.Frontiers in cellular neuroscience · 2019Article
- A Versatile and Open-Source Rapid LED Switching System for One-Photon Imaging and Photo-Activation.Frontiers in cellular neuroscience · 2018Article
- Simultaneous recordings of voltage and current waveforms from dendrites.The Journal of physiology · 2016Article
- Functional coupling of diverse voltage-gated Ca(2+) channels underlies high fidelity of fast dendritic Ca(2+) signals during burst firing.The Journal of physiology · 2016Article
- Optical recording of calcium currents during impulse conduction in cardiac tissue.Neurophotonics · 2015Article
- Using simultaneous voltage and calcium imaging to study fast Ca(2+) channels.Neurophotonics · 2015Article
- Combining Membrane Potential Imaging with Other Optical Techniques.Advances in experimental medicine and biology · 2015Review
- Shift and Mean Algorithm for Functional Imaging with High Spatio-Temporal Resolution.Frontiers in cellular neuroscience · 2015Article
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Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current understanding of Ca(2+) channel function is derived from the use of the patch-clamp technique. In particular, the measurement of fast cellular Ca(2+) currents is routinely achieved using whole-cell voltage-clamp recordings. However, this experimental approach is not applicable to the study of local native Ca(2+) channels during physiological changes of membrane potential in complex cells, since the voltage-clamp configuration constrains the membrane potential to a given value. Here, we report for the first time to our knowledge that Ca(2+) currents from individual cells can be quantitatively measured beyond the limitations of the voltage-clamp approach using fast Ca(2+) imaging with low-affinity indicators. The optical measurement of the Ca(2+) current was correlated with the membrane potential, simultaneously measured with a voltage-sensitive dye to investigate the activation of Ca(2+) channels along the apical dendrite of the CA1 hippocampal pyramidal neuron during the back-propagation of an action potential. To validate the method, we analyzed the voltage dependence of high- and low-voltage-gated Ca(2+) channels. In particular, we measured the Ca(2+) current component mediated by T-type channels, and we investigated the mechanisms of recovery from inactivation of these channels. This method is expected to become a reference approach to investigate Ca(2+) channels in their native physiological environment.
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