ArticleBoneKEy reports2014
Osteocyte expression of caspase-3, COX-2, IL-6 and sclerostin are spatially and temporally associated following stress fracture initiation.
Article in BoneKEy reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Oxygen-dependent osteocyte transcriptomic adaptations occur without modulation of endothelial gene expression via osteocyte paracrine signaling.JBMR plus · 2026Article
- Unraveling stress fractures: from cellular and molecular mechanisms to therapeutic approaches.Journal of orthopaedic translation · 2026Review
- Osteocyte-vascular Interactions in Bone Physiology and Pathology.Current osteoporosis reports · 2026Review
- Unexpected periosteal bone apposition including newly embedded osteocytes occurs around a mouse calvaria critical defect, independently of the presence of biomaterials.Scientific reports · 2026Article
- Sclerostin's role in bone-muscle crosstalk and osteoporosis pathogenesis.Osteoporosis and sarcopenia · 2025Review
- Fracture Healing in the Setting of Endocrine Diseases, Aging, and Cellular Senescence.Endocrine reviews · 2022Review
- Finite Element Models of Osteocytes and Their Load-Induced Activation.Current osteoporosis reports · 2022Review
- Generalized Uncoupled Bone Remodeling Associated With Delayed Healing of Fatigue Fractures.JBMR plus · 2022Article
- Histopathological Finding of Microdamage Accumulation in Atypical Subtrochanteric Femoral Fracture.Case reports in orthopedics · 2021Article
- How much do we know about the role of osteocytes in different phases of fracture healing? A systematic review.Journal of orthopaedic translation · 2020Review
- Osteoblast and Osteoclast Activity Affect Bone Remodeling Upon Regulation by Mechanical Loading-Induced Leukemia Inhibitory Factor Expression in Osteocytes.Frontiers in molecular biosciences · 2020Article
- Monocyte Chemoattractant Protein-1 (MCP-1/CCL2) Drives Activation of Bone Remodelling and Skeletal Metastasis.Current osteoporosis reports · 2019Review
- IL-6 exhibits bothThe Journal of biological chemistry · 2019Article
- Osterix regulates corticalization for longitudinal bone growth via integrin β3 expression.Experimental & molecular medicine · 2018Article
- Reg Gene Expression in Periosteum after Fracture and Its In Vitro Induction Triggered by IL-6.International journal of molecular sciences · 2017Article
- Article
- Connecting the Dots: Linking Osteocyte Activity and Therapeutic Modulation of Sclerostin by Extending a Multiscale Systems Model.CPT: pharmacometrics & systems pharmacology · 2015Article
- Mechanical loading reduces inflammation-induced human osteocyte-to-osteoclast communication.Calcified tissue international · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stress fractures (SFxs) are debilitating injuries and exact mechanisms that initiate their repair incompletely understood. We hypothesised that osteocyte apoptosis and expression of cytokines and proteins such as sclerostin, VEGF, TGF-β, COX-2 and IL-6 were early signalling events to facilitate the formation of periosteal woven bone and recruitment of osteoclast precursors to the site of remodelling. A SFx was created in the right ulna of mature female wistar rats using cyclic end loading. Rats were killed 1, 4 and 7 days after loading (n=5 per group). Standard histological staining was used to examine SFx morphology and immunohistochemistry to detect the localisation of these proteins and in situ hybridisation to detect mRNA along the SFx line or gene expression to quantify the target genes. Unloaded ulnae served as controls. The labelling index of caspase-3, COX-2 and IL-6 was significantly elevated in the region of SFxs at all time points compared with controls (P<0.001). In addition, the labelling index of sclerostin protein was significantly reduced in osteocytes adjacent to the SFx region when compared with controls at all three time points (P<0.001). Both VEGF and TGF-β expressions were only localised in the woven bone. These data reinforce the involvement of osteocyte apoptosis in the healing of fatigue damage in bone, and demonstrate that local regulation of sclerostin, COX-2 and IL-6 are important signalling events associated with new bone formation and SFx remodelling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.