ArticleFrontiers in plant science2014
The F130S point mutation in the Arabidopsis high-affinity K(+) transporter AtHAK5 increases K(+) over Na(+) and Cs(+) selectivity and confers Na(+) and Cs(+) tolerance to yeast under heterologous expression.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- The superior growth of Kluyveromyces marxianus at very low potassium concentrations is enabled by the high-affinity potassium transporter Hak1.FEMS yeast research · 2024Article
- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- Article
- Potassium transporter KUP9 participates in KStress biology · 2022Article
- Genome Editing Targets for Improving Nutrient Use Efficiency and Nutrient Stress Adaptation.Frontiers in genetics · 2022Review
- Article
- An endoplasmic reticulum-localized cytochromeProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Regulation of Cytosolic pH: The Contributions of Plant Plasma Membrane HInternational journal of molecular sciences · 2021Review
- Genome-Wide Analysis ofGenes · 2021Article
- The role of PQL genes in response to salinity tolerance inPlant direct · 2021Article
- Genome-wide characterization and expression analysis of HAK K3 Biotech · 2021Article
- HalophyticInternational journal of molecular sciences · 2020Article
- Recognition and Activation of the Plant AKT1 Potassium Channel by the Kinase CIPK23.Plant physiology · 2020Article
- Structural basis of proton-coupled potassium transport in the KUP family.Nature communications · 2020Article
- Molecular Evolution and Expansion of the KUP Family in the Allopolyploid Cotton SpeciesFrontiers in plant science · 2020Article
- The Dynamics of Radio-Cesium in Soils and Mechanism of Cesium Uptake Into Higher Plants: Newly Elucidated Mechanism of Cesium Uptake Into Rice Plants.Frontiers in plant science · 2020Review
- Amelioration of the Oxidative Stress Generated by Simple or Combined Abiotic Stress through the K⁺ and CaAntioxidants (Basel, Switzerland) · 2019Article
- Regulation of KFrontiers in plant science · 2019Review
- Foxtail millet SiHAK1 excites extreme high-affinity KPlant cell reports · 2018Article
- Review
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6 authors.
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Abstract
Potassium (K(+)) is an essential macronutrient required for plant growth, development and high yield production of crops. Members of group I of the KT/HAK/KUP family of transporters, such as HAK5, are key components for K(+) acquisition by plant roots at low external K(+) concentrations. Certain abiotic stress conditions such as salinity or Cs(+)-polluted soils may jeopardize plant K(+) nutrition because HAK5-mediated K(+) transport is inhibited by Na(+) and Cs(+). Here, by screening in yeast a randomly-mutated collection of AtHAK5 transporters, a new mutation in AtHAK5 sequence is identified that greatly increases Na(+) tolerance. The single point mutation F130S, affecting an amino acid residue conserved in HAK5 transporters from several species, confers high salt tolerance, as well as Cs(+) tolerance. This mutation increases more than 100-fold the affinity of AtHAK5 for K(+) and reduces the K i values for Na(+) and Cs(+), suggesting that the F130 residue may contribute to the structure of the pore region involved in K(+) binding. In addition, this mutation increases the V max for K(+). All this changes occur without increasing the amount of the AtHAK5 protein in yeast and support the idea that this residue is contributing to shape the selectivity filter of the AtHAK5 transporter.
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